PPP1CA ELISA Kit (Bovine)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

PPP1CA

Store as indicated in product manual.

NM_001035316

Q3T0E7

Assay Methodology: Quantitative Competitive ELISA

Aviva Systems Biology Ppp1ca ELISA Kit (Bovine) (OKEH06691) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ppp1ca has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ppp1ca is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ppp1ca captured in the well.

Serum, Plasma, Tissue Homogenates