« Back
Home /
PPP1CA ELISA Kit (Bovine)

PPP1CA ELISA Kit (Bovine)

Item no.	OKEH06691
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Cell Biology Cell Status ELISA Senescence ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Cow (Bos taurus), Cattle (Bovine)
Sensitivity	0.25 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	PP-1A;serine/threonine-protein phosphatase PP1-alpha catalytic subunit.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Competitive
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	PPP1CA
Gene Fullname	protein phosphatase 1 catalytic subunit alpha
Reconstitution and storage	Store as indicated in product manual.
Description of target	Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
Nucleotide accession_num	NM_001035316
Protein accession_num	NP_001030393
Protein name	Q3T0E7
Manufacturer - Specificity	Natural and recombinant Bovine Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
Assay info	Assay Methodology: Quantitative Competitive ELISA
Kit duration	~3 Hours
Kit principle	Aviva Systems Biology Ppp1ca ELISA Kit (Bovine) (OKEH06691) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ppp1ca has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ppp1ca is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ppp1ca captured in the well.
Kit reproducibility	Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates. Intra-Assay CV <10% Inter-Assay CV <12%
Sample Type	Serum, Plasma, Tissue Homogenates

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKEH06691.pdf

OKEH06691-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: 96 Wells

available

Delivery expected until 10/30/2025

Express Delivery: Save 1 days

Compare

Add to wishlist

Get an offer