Vergleich

PDAP1 ELISA Kit (Human)

ArtNr OKEI00177
Hersteller AVIVA Systems Biology
Menge 96 Wells
Kategorie
Typ Elisa-Kit
Specific against Human (Homo sapiens)
Sensitivity 0.469 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias PAP,PAP1,HASPP28
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
PDAP1
Reconstitution and storage
Store as indicated in product manual.
Description of target
Enhances PDGFA-stimulated cell growth in fibroblasts, but inhibits the mitogenic effect of PDGFB.By similarity
Nucleotide accession_num
NM_014891
Protein accession_num
NP_055706
Protein name
28 kDa heat- and acid-stable phosphoprotein
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit duration
~ 3 Hours
Kit principle
Aviva Systems Biology PDAP1 ELISA Kit (Human) (OKEI00177) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PDAP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PDAP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample PDAP1
Kit reproducibility
Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level PDAP1. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level PDAP1 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
Sample Type
Serum, Plasma, Biological Fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 Wells
Lieferbar: In stock
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