Comparison

PDAP1 ELISA Kit (Human)

Item no. OKEI00177
Manufacturer AVIVA Systems Biology
Amount 96 Wells
Category
Type Elisa-Kit
Specific against Human (Homo sapiens)
Sensitivity 0.469 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias PAP,PAP1,HASPP28
Shipping Condition Cool pack
Available
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
PDAP1
Reconstitution and storage
Store as indicated in product manual.
Description of target
Enhances PDGFA-stimulated cell growth in fibroblasts, but inhibits the mitogenic effect of PDGFB.By similarity
Nucleotide accession_num
NM_014891
Protein accession_num
NP_055706
Protein name
28 kDa heat- and acid-stable phosphoprotein
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit duration
~ 3 Hours
Kit principle
Aviva Systems Biology PDAP1 ELISA Kit (Human) (OKEI00177) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PDAP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PDAP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample PDAP1
Kit reproducibility
Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level PDAP1. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level PDAP1 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
Sample Type
Serum, Plasma, Biological Fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 Wells
Available: In stock
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