SEMA4C ELISA Kit (Human)

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SEMA4C

Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration.

NP_060259

Assay Methodology: Quantitative Sandwich ELISA

Aviva Systems Biology SEMA4C ELISA Kit (Human) (OKEI00291) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SEMA4C has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SEMA4C is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample SEMA4C

Serum, Plasma, Biological Fluids