IL12B ELISA Kit (Rat)

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IL12B

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.

XP_006246209

Assay Methodology: Quantitative Sandwich ELISA

Aviva Systems Biology Il12b ELISA Kit (Rat) (OKEI00681) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Il12b has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Il12b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Il12b

Serum, Plasma, Biological Fluids