SPAG5 ELISA Kit (Human)

Enzyme-linked Immunosorbent assay-Sandwich

Wet Ice

sperm associated antigen 5

Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase (PubMed:11724960, PubMed:12356910, PubMed:27462074). Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture (PubMed:17664331, PubMed:27462074). In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes (PubMed:18361916, PubMed:21402792). Involved in centriole duplication. Required for CDK5RAP2, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2 (PubMed:26297806). In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation-induced apoptosis (PubMed:23953116). May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU (PubMed:18055457).

NP_006452

Assay Type: Quantitative Sandwich ELISA

Colorimetric, OD 450 nm

Sample Type - 1:2 Dilution - 1:4 Dilution - 1:8 Dilution - 1:16 Dilution
Serum (n=5) - 87-103% - 91-96% - 88-100% - 88-102%
EDTA Plasma (n=5) - 82-98% - 86-101% - 88-101% - 86-100%
Heparin Plasma (n=5) - 91-98% - 85-98% - 84-96% - 81-100%

Sample Type - Range - Mean
Serum (n=5) - 86-100 - 94
EDTA Plasma (n=5) - 90-95 - 93
Heparin Plasma (n=5) - 86-104 - 95

Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids