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SPAG5 ELISA Kit (Human)

SPAG5 ELISA Kit (Human)

Item no.	OKWB00200
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Oncology Neuroscience Molecular Targets for Neuroscience Targets for Rodent Neurosciences Aging & Neurological Disorders Protein Aggregation, Clearance & Misfolding By Cell Type Carcinoma Hematology Leukemia Lymphoma Myeloma Sarcoma Cell Biology Cell Status Adenocarcinoma ELISA Autophagy ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	0.094 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	astrin;DEEPEST;hMAP126;MAP126;mitotic spindle associated protein p126;mitotic spindle coiled-coil related protein;Mitotic spindle-associated protein p126;sperm-associated antigen 5.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	SPAG5
Gene Fullname	sperm associated antigen 5
Reconstitution and storage	Store as indicated in product manual.
Description of target	Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase (PubMed:11724960, PubMed:12356910, PubMed:27462074). Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture (PubMed:17664331, PubMed:27462074). In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes (PubMed:18361916, PubMed:21402792). Involved in centriole duplication. Required for CDK5RAP2, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2 (PubMed:26297806). In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation-induced apoptosis (PubMed:23953116). May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU (PubMed:18055457).
Nucleotide accession_num	NM_006461
Protein accession_num	NP_006452
Protein name	Sperm-associated antigen 5
Assay info	Assay Type: Quantitative Sandwich ELISA
Kit Components	Component: Amount SPAG5 Microplate: 96 Wells (12 x 8 Well strips) SPAG5 Lyophilized Standard: 2 x 10 ng 100X SPAG5 Biotinylated Detector Anitbody: 1 x 120 uL 100X Avidin-HRP Conjugate: 1 x 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 25X Wash Buffer: 1 x 20 mL TMB Substrate: 1 x 10 mL Stop Solution: 1 x 10 mL
Kit detection	Colorimetric, OD 450 nm
Kit duration	~ 3 Hours
Kit linearity	Sample Type - 1:2 Dilution - 1:4 Dilution - 1:8 Dilution - 1:16 Dilution Serum (n=5) - 87-103% - 91-96% - 88-100% - 88-102% EDTA Plasma (n=5) - 82-98% - 86-101% - 88-101% - 86-100% Heparin Plasma (n=5) - 91-98% - 85-98% - 84-96% - 81-100%
Kit principle	Aviva Systems Biology SPAG5 ELISA Kit (Human) (OKWB00200) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SPAG5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SPAG5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SPAG5 captured in the well.
Kit recovery	Sample Type - Range - Mean Serum (n=5) - 86-100 - 94 EDTA Plasma (n=5) - 90-95 - 93 Heparin Plasma (n=5) - 86-104 - 95
Kit reproducibility	Mean Intra-Assay Precision <10% Mean Inter-Assay Precision <12%
Sample Type	Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96 Wells

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Delivery expected until 12/25/2025

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