Collagen I, rat tail (Rat)

Protein

Wet Ice

Can be used for preparation of collagen gels, thin layer coating of surfaces (culture plates, slides, coverslips).

Liquid 0.02N acetic acid
Physical Appearance: Hazy viscous liquid

Collagen is the main component in connective tissue and helps to provide support for tissues. It is made up of several classes, with Type 1 collagen being the most common. Type 1 collagen has a herterotrimeric triple helical structure made up of two alpha-1(I) and one alpha-2(I) chains that form into elongated fibrils which are extremely strong. These fibrils can be found in skin, tendons, ligaments, and other connective tissues. Type 1 collagen has been shown to be useful as a substrate that promotes cell growth and proliferation. Under acidic conditions the protein is soluble, however by raising the temperature and pH the solution forms into a solid gel that can be useful for cellular studies. It can also be dried to form a thin layer on solid surfaces such as plates, slides, or coverslips to aid in cell attachment.

NP_002147.2

Tested in cell proliferation assay - Increased attachment of cells on collagen coated coverslips.

5 mg/ml