b NGF Protein (OPPA00511)

b NGF CHO

The protein was lyophilized from a 0.2Aum filtered solution in 20mM PB and 250mM NaCl, pH 7.0. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

It is recommended to reconstitute the lyophilized NGF-b in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Lyophilized Nerve Growth Factor b although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Nerve Growth Factor-beta should be stored at 4C between 2-7 days and for future use below -18C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

The ED₅₀, calculated by its ability to stimulate chick E9 DRG neurite outgrowth was found to be < 1.0 ng/ml, corresponding to a specific activity of > 1 x 10⁶ units/mg.