b NGF Protein (OPPA00511)

Hersteller AVIVA Systems Biology
Kategorie
Typ Proteins
Specific against other
Format The protein was lyophilized from a 0.2 um filtered solution in 30mM sodium acetate pH=5.5 containing 1% HSA. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Menge 5ug
Host Hamster
ArtNr OPPA00511
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Description
Nerve Growth Factor-beta Human Recombinant produced in CHO is a noncovalently disulfide linked homodimer, glycosylated, polypeptide chain (Ser122-Arg239) containing 2 identical 118 amino acids and having a molecular mass of 26.5 kDa.
Gene symbol
b NGF CHO
Protein name
Beta-nerve growth factor
Product format
The protein was lyophilized from a 0.2Aum filtered solution in 20mM PB and 250mM NaCl, pH 7.0. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Gene id
4803
Reconstitution and storage
It is recommended to reconstitute the lyophilized NGF-b in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Lyophilized Nerve Growth Factor b although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Nerve Growth Factor-beta should be stored at 4C between 2-7 days and for future use below -18C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Description of target
NGF-beta has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis.
Biological activity
The ED50, calculated by its ability to stimulate chick E9 DRG neurite outgrowth was found to be < 1.0 ng/ml, corresponding to a specific activity of > 1 x 106 units/mg.
Purity
Greater than 95.0% as determined by
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.

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Menge: 5ug
Lieferbar: In stock
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