ArtNr |
OPPA02003 |
Hersteller |
AVIVA Systems Biology
|
Menge |
2ug |
Kategorie |
|
Typ |
Proteins |
Format |
Leukemia Inhibitory Factor (LIF) was lyophilized from a concentrated (1mg/ml) sterile solution containing 1x PBS. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder. |
Specific against |
other |
Host |
E.coli |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
CDF, HILDA, D-FACTOR, Differentiation- stimulating factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin, Leukemia inhibitory factor, LIF, DIA, LIF Human, Leukemia Inhibitory Factor Human Recombinant |
Similar products |
LIF |
Lieferbar |
|
Gene symbol |
LIF |
Protein name |
Leukemia inhibitory factor |
Protein size |
180 |
Molecular weight |
19.7 kDa |
Product format |
Lyophilized from a concentrated (1mg/ml) solution in water containing 1x PBS, pH 7.4. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder. |
Gene id |
3976 |
Reconstitution and storage |
Reconstitution: It is recommended to reconstitute the lyophilized Leukemia Inhibitory Factor (LIF) in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions. Storage: Lyophilized Leukemia Inhibitory Factor (LIF) although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Leukemia Inhibitory Factor (LIF) should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Description of target |
Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. |
Purification |
The Leukemia Inhibitory Factor (LIF) is purified by proprietary chromatographic techniques. |
Biological activity |
The ED50 was determined by the M1 cell differentiation assay is < 0.01 ng/ml, corresponding to a specific activity of 100, 000, 000IU/mg. |
Purity |
Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
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