Item no. |
OPPA02003 |
Manufacturer |
AVIVA Systems Biology
|
Amount |
2ug |
Category |
|
Type |
Proteins |
Format |
Leukemia Inhibitory Factor (LIF) was lyophilized from a concentrated (1mg/ml) sterile solution containing 1x PBS. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder. |
Specific against |
other |
Host |
E.coli |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
CDF, HILDA, D-FACTOR, Differentiation- stimulating factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin, Leukemia inhibitory factor, LIF, DIA, LIF Human, Leukemia Inhibitory Factor Human Recombinant |
Similar products |
LIF |
Available |
|
Gene symbol |
LIF |
Protein name |
Leukemia inhibitory factor |
Protein size |
180 |
Molecular weight |
19.7 kDa |
Product format |
Lyophilized from a concentrated (1mg/ml) solution in water containing 1x PBS, pH 7.4. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder. |
Gene id |
3976 |
Reconstitution and storage |
Reconstitution: It is recommended to reconstitute the lyophilized Leukemia Inhibitory Factor (LIF) in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions. Storage: Lyophilized Leukemia Inhibitory Factor (LIF) although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Leukemia Inhibitory Factor (LIF) should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Description of target |
Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. |
Purification |
The Leukemia Inhibitory Factor (LIF) is purified by proprietary chromatographic techniques. |
Biological activity |
The ED50 was determined by the M1 cell differentiation assay is < 0.01 ng/ml, corresponding to a specific activity of 100, 000, 000IU/mg. |
Purity |
Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.