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Human beta-Glucuronidase/GUSB Protein, His Tag (active enzyme)

Human beta-Glucuronidase/GUSB Protein, His Tag (active enzyme)

ArtNr	BEB-H52H3-1mg
Hersteller	ACROBiosystems
Menge	500 ug x 2
Quantity options	100 ug 500 ug x 2
Kategorie	Peptides & Proteins Recombinant Proteins
Typ	Proteins Recombinant
Format	Solid
Specific against	Human (Homo sapiens)
Host	HEK293
Konjugat/Tag	Unconjugated, HIS
Purity	95%
Formula	50 mM Tris,150 mM NaCI,pH7.5
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Versandbedingung	Raumtemperatur
Lieferbar
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	C-10xHis, Unconjugated
Shipping Temperature	RT
Storage Conditions	-20°C
Molecular Weight	74.5 kDa
Manufacturer - Format	Powder
Description	Human beta-Glucuronidase Protein, His Tag (BEB-H52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Thr 651 (Accession # P08236).
Background	Human beta -Glucuronidase (EC 3.2.1.31) encoded by the GUSB gene is a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans that include heparan sulfate, chondroitin sulfate and hyaluronan. The enzyme is only active on the glucuronic acid of the non-reducing end. The native protein has been reported as a tetrameric glycoprotein composed of identical subunits.
Molecule	beta-Glucuronidase/GUSB
Exp Region	Leu 23 - Thr 651
Characteristics	This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 74.5 kDa. The protein migrates as 75-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin	1.0 EU per μg
Buffer	50 mM Tris, 150 mM NaCI, pH 7.5
Stability	● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant	trehalose

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Arbeitsanleitung (Data Sheet) anfordern

BEB-H52H3-1mg-datasheet.pdf

BEB-H52H3-1mg-safety-datasheet.pdf