QuantiChrom™ Alcohol dehydrogenase Assay Kit

RT

For quantitative determination of alcohol dehydrogenase activity and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (20 µ L sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Biological (e.g. plasma, serum, urine, tissue and culture media). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.4 U/L.

Application: For quantitative determination of alcohol dehydrogenase activity and evaluation of drug effects on its metabolism.
Key Features: Fast and sensitive. Linear detection range (20 ?L sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 min reaction.
Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method: OD565nm
Samples: Biological (e.g. plasma, serum, urine, tissue and culture media)
Species: All
Size: 100 tests
Detection Limit: 0.4 U/L
Shelf Life: 6 months
More Details: ALCOHOL DEHYDROGENASE (ADH) is an oxidoreductase which catalyzes the interconversion of alcohols and aldehydes or ketones. ADH is important in humans and other organisms for the break down of alcohols which may otherwise be toxic. In yeast and some bacteria, ADHs catalyze the opposite reaction and produce alcohol as part of fermentation. BioAssay Systems non-radioactive, colorimetric ADH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.