QuantiChrom™ β-Glucosidase Assay Kit

RT

For quantitative determination of β -glucosidase activity and evaluation of drug effects on its metabolism. Key Features: High sensitivity and wide linear range. Use 20 µ L sample. The detection limit is 2 U/L, linear up to 250 U/L. Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of β -glucosidase activity within 20 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Method: OD405nm. Samples: Biological. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 2 U/L.

Application: For quantitative determination of ?-glucosidase activity and evaluation of drug effects on its metabolism.
Key Features: High sensitivity and wide linear range. Use 20 ?L sample. The detection limit is 2 U/L, linear up to 250 U/L.
Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of ?-glucosidase activity within 20 minutes.
Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.
Method: OD405nm
Samples: Biological
Species: All
Size: 100 tests
Detection Limit: 2 U/L
Shelf Life: 6 months
More Details: ?-GLUCOSIDASE is a glucosidase enzyme which acts upon ?1-> 4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). ?-Glucosidases are required by organisms (some fungi, bacteria, termites) for consumption of cellulose. Lysozyme is also a ?-glucosidase and is present in tears to prevent bacterial infection of the eye. In humans, lower activity of a ?-glucosidase isoform (lysosomal gluco-cerebrosidase) has been related to Gaucher’s disease and Parkinson’s disease. Simple, direct and automation-ready procedures for measuring ?-glucosidase activity are becoming popular in Research and Drug Discovery. BioAssay Systems QuantiChrom ?-Glucosidase Assay Kit is designed to measure ?-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-?-D-glucopyranoside that is hydrolyzed specifically by ?-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.