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QuantiChrom Carbonyl Assay Kit

QuantiChrom Carbonyl Assay Kit

ArtNr	BAS-DCAR-100
Hersteller	BioAssay Systems
Menge	100 Tests
Kategorie	Other Assays & Kits Other Kits
Typ	Assay-Kit
Specific against	other
Citations	Pena-Bermudez, Y. A., et al. (2020). Effects of feeding increasing levels of yerba mate on lamb meat quality and antioxidant activity. Animals: An Open Access Journal from MDPI 10(9). Assay: Carbonyl Groups in lamb longissimus thoracis muscle. To find more recent publications, please click here.
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Lieferbar
Description	Quantification of carbonyl groups or protein carbonyls in biological samples
Faqs	The background is very high. Is this normal? The background absorbance is typically ca. 0.5. This is completely normal. Can I store the diluted standards (250 uM, 150 uM, 75 uM, and 0 uM) to use for later? We recommend preparing the standards fresh from the 50 mM each day. Do I need to run a standard curve with every assay? Yes. How should I store the kit? The kit is shipped at room temperature. Upon delivery, store at -20C. Precipitate formed when I added the reagent to the sample. What should I do? The Reagent is acidic and may cause precipitation in samples that have significant amounts of protein. You may need to dilute your sample, repeat the assay, and correct for the dilution factor in the calculation. What is the Carbonyl Standard? The standard is pyruvate, which has two carbonyl groups per molecule. So the 50 mM Carbonyl Standard is actually 25 mM pyruvate. My sample has high levels of nucleic acids. Will this interfere? Nucleic acids can artificially increase the carbonyl count if they are present in high levels. If the ratio of 280/260 nm is less than 1, samples should be mixed with 1% streptomycin sulfate to denature the nucleic acids. Mix well, incubate the samples at room temperature for 15 minutes and then centrifuge at 5000xg for 10 minutes. Use the supernatant in the assay. For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Overview	Application Quantification of carbonyl groups or protein carbonyls in biological samples. Key Features Fast and sensitive. Use of 100 uL sample. Linear detection range from 12 to 250 & microM carbonyl in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 min. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method OD375nm Samples Chemicals with carbonyl groups (e.g. ketones, aldehydes) or protein carbonyls in biological samples (e.g. oxidized BSA, etc.) Species All Size 100 tests Detection Limit 12 uM Shelf Life 12 months More Details CARBONYL groups, such as ketones and aldehydes, are a commonindicator of protein oxidation. Protein oxidation is caused as a result of exposure to reactive oxygen species (ROS) and is a common marker in various diseases as well as aging. BioAssay Systems' Carbonyl Assay Kit is based on an improved method, where 2, 4-dinitrophenylhydrazine (DNPH) reacts with carbonyl groups to produce a colored compound at 375 nm. The intensity of this colored compound is directly proportional to the carbonyl groups in the sample.
Shipment	RT
Storage	-20 C

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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BAS-DCAR-100-datasheet.pdf

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Menge: 100 Tests

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