QuantiChrom™ Sialic Acid Assay Kit

RT

For quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on sialic acid. Key Features: Sensitive and accurate. Use as little as 60 µ L samples. Linear detection range in 96-well plate: 5 to 1000 µ M sialic acid for colorimetric assays and 0.5 to 100 µ M for fluorimetric assays. Method: OD549nm, or FL555/585nm. Samples: Serum, plasma, saliva, milk etc. Species: All. Procedure: Assay takes 2.5 hrs. Kit size: 100 tests. Detection limit: OD, FL: 5, 0.5 µ M.

Application: For quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on sialic acid.
Key Features: Sensitive and accurate. Use as little as 60 ?L samples. Linear detection range in 96-well plate: 5 to 1000 ?M sialic acid for colorimetric assays and 0.5 to 100 ?M for fluorimetric assays.
Method: OD549nm, or FL555/585nm
Samples: Serum, plasma, saliva, milk etc
Species: All
Size: 100 tests
Detection Limit: OD, FL: 5, 0.5 ?M
Shelf Life: 24 months
More Details: SIALIC ACID is a general name for nine carbon acidic sugars with N- or O-substituted derivatives. The most common member of these sugars is N-acetylneuraminic acid (NANA). Sialic acid is widely distributed throughout mammalian tissues and fluids including serum. Sialylated oligosaccharides have been shown to exhibit antiviral properties and are also known to influence blood coagulation and cholesterol levels. The sialic acid level in body fluids is also an important marker for diagnosing cancer. Simple and direct procedures for measuring sialic acid concentrations find wide applications in research and drug discovery. BioAssay Systems sialic acid assay uses an improved Warren method, in which sialic acid is oxidized to formylpyruvic acid which reacts with thiobarbituric acid to form a pink colored product. The color intensity at 549 nm or fluorescence intensity at ?ex/em = 555/585 nm is directly proportional to sialic acid concentration in the sample.