Vergleich

EnzyChrom AF HDL and LDL/VLDL Assay Kit

Hersteller BioAssay Systems
Kategorie
Typ Assay-Kit
Specific against other
Menge 100 Tests
ArtNr BAS-E2HL-100
Targets APOB;;HSD11B1
eClass 6.1 32161000
eClass 9.0 32161090
Lieferbar
Description
Quantitative determination of HDL and LDL/VLDL cholesterol by colorimetric (570nm) or fluorimetric (530nm/590nm) methods. Procedure: 60 min. Kit size: 100 tests. Detection limit: colorimetric assay 1 mg/dL, fluorimetric assay 0.2 mg/dL. Shelf life: 6 mont
Faqs

I want to ask about the differences between "EnzyChrom AF HDL and LDL/VLDL Assay Kit and EnzyChrom HDL and LDL/VLDL Assay Kit. Since I want to work on mice.

Both kits will work with mouse serum and plasma samples, but I would recommend using serum, because we have not validated the use of plasma. The EHDL-100 kit has a linear detection range of 5 -300 mg/dL. It requires a microplate reader with a 340nm filter. The E2HL-100 kit is more sensitive with a linear detection range of 1 -100 mg/dL using the colorimetric readout (requires a 570 nm filter), or 0.2 -10 mg/dL with the fluorimetric readout (requires a fluorescence microplate reader).


For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Overview

Application

  • For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism.

Key Features

  • Sensitive and accurate. Linear detection range in 96-well plate: 1 to 100 mg/dL cholesterol for colorimetric assays and 0.2 to 10 mg/dL for fluorimetric assays.
  • Convenient. Room temperature assay. No 37C heater is needed.

Method

  • OD570nm, or FL530/585nm

Samples

  • Serum

Species

  • All

Size

  • 100 tests

Detection Limit

  • OD, FL: 1, 0.2 mg/dL

Shelf Life

  • 6 months

More Details

  • CHOLESTEROL concentrations in High-Density Lipoprotein (HDL) and Low-Density (LDL)/Very-Low-Density (VLDL) Lipoproteins are strong predictors for coronary heart disease. Functional HDL offers protection by removing cholesterol from cells and atheroma. Higher concentrations of LDL and lower concentrations of functional HDL are strongly associated with cardiovascular disease due to higher risk of atherosclerosis. The balances between high- and low-density lipoproteins are solely genetically determined, but can be changed by medications, food choices and other factors. Simple, direct and automation-ready procedures for measuring HDL and LDL/VLDL concentrations are very desirable. BioAssay Systems HDL and LDL/VLDL quantification kit is based on our improved PEG precipitation method in which HDL and LDL/VLDL are separated, and cholesterol concentrations are determined using a single Working Reagent that combines cholesterol ester hydrolysis, oxidation and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at ?ex/em = 530/585nm is directly proportional to total cholesterol concentration in the sample.
Shipment
ICE
Storage
-20 C

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 Tests
Lieferbar: In stock
lieferbar

Lieferung vsl. bis 25.04.2024 

Vergleichen

Auf den Wunschzettel

Angebot anfordern

Lieferzeit anfragen

Technische Frage stellen

Bulk-Anfrage stellen

Fragen zum Produkt?
 
Schließen