EnzyChrom™ Coenzyme A Assay Kit

ICE

For quantitative determination of coenzyme A (CoA) and evaluation of drug effects on CoA metabolism. Key Features: Sensitive. Use 10 µ L samples. Linear detection range: colorimetric assay 5 - 1000 µ M, fluorimetric assay 3 - 100 µ M CoA. Convenient. Room temperature "mix-and-read" procedure can be readily automated for high-throughput assay of thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 3 µ M.

Application: For quantitative determination of coenzyme A (CoA) and evaluation of drug effects on CoA metabolism.
Key Features: Sensitive. Use 10 ?L samples. Linear detection range: colorimetric assay 5 - 1000 ?M, fluorimetric assay 3 - 100 ?M CoA.
Convenient. Room temperature "mix-and-read" procedure can be readily automated for high-throughput assay of thousands of samples per day.
Method: OD570nm, or FL530/585nm
Samples: Biological
Species: All
Size: 100 tests
Detection Limit: 3 ?M
Shelf Life: 6 months
More Details: Coenzyme A (CoA) is involved in many important biological activities including the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many others. One of CoA’s most crucial roles is the carrying and transferring of acyl groups. BioAssay Systems method provides a simple, two-step and high-throughput assay for measuring CoA. In this assay, the first step enzymatically converts CoA to acyl-CoA and the second step oxidizes the acyl-CoA producing an enoyl-CoA and H₂O₂. The resulting H₂O₂ reacts with a specific dye to form a pink colored product. The optical density at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the CoA concentration in the sample.