EnzyFluo Isocitrate Assay Kit

Application

• For quantitative determination of isocitrate and evaluation of drug effects on its metabolism.

Key Features

• Fast and sensitive. Linear detection range (20 ?L sample): 0.6 to 500 ?M for 10 min reaction.
• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• FL530/585 nm

Samples

• Food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)

Species

• All

Size

• 100 tests

Detection Limit

• 0.6 uM

Shelf Life

• 6 months

More Details

• ISOCITRATE (ISOCITRIC ACID) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing ?-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices. BioAssay Systems' isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at ?ex/em = 530/585 nm, is proportional to the isocitrate concentration in the sample.