EnzyChrom Histamine Assay Kit

Does the assay kit work in particular species?

Our assays work in all species. None of our kits are species specific.

Do you offer free sample kits or discounts for testing?

Unfortunately, at this time we do not offer free sample kits. Customers must purchase assay kits for their own testing, on occasion we will offer discounts.

Does the histamine kit suitable for biological samples such as blood, urine, etc.?

Since most of the biological samples contain histamine under the kit detection limit, this kit is only suitable for food, beverage, and agricultural products.

How do I prepare the solid & liquid samples?

Fish or other solid samples: Weigh ˜1 g sample and grind to paste. Weigh ˜200mg paste sample and homogenize in 800 ?L 0.05% acetic acid. May vortex or use handheld homogenzier to homogenize sample. If using blender to homogenize sample, just simply scale up the sample size and solvent in 1mg: 4 ?L ratio (ex: 5 g samples in 20 mL acid). Centrifuge homogenate at 14, 000 g for 10 min. Transfer the clear supernatant into a clean tube. It is prudent to run a pilot test of the sample at different dilutions. Choose a dilution with the readings in the detection range of the standard curve for further assays. Most samples can be stored at -80C if not assayed immediately.

Cheese: Weigh ˜200 mg sample and homogenzie in 800 ?L 0.05% acetic acid. Heat in heat bath at 60C for 10mins. Centrifuge homogenate at 14, 000 g for 10 min. Transfer the clear supernatant into a clean tube.

Liquid samples: Fish sauce or soy sauce: 1:5 dilution in H₂O. Red wine or other alcoholic beverage: No dilution necessary.

Can I store unused reagents for future use?

Yes, unused reagents can be stored according to the assay protocol. Repeated freeze/thaw cycles of reagents should be avoided.

Do I need to use a standard or standard curve with each assay run?

Yes, it is highly recommended to run for each assay a standard curve, or where applicable, internal standard.


For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.

ICE