EnzyChrom™ Malate Dehydrogenase Assay Kit

ICE

For quantitative determination of L-malate dehydrogenase enzyme activity and drug effects on malate dehydrogenase metabolism. Key Features: Fast and sensitive. Linear detection range (20 µ L sample): 0.5 to 65 U/L for 20 min reaction at 37° C. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.5 U/L.

Application: For quantitative determination of L-malate dehydrogenase enzyme activity and drug effects on malate dehydrogenase metabolism.
Key Features: Fast and sensitive. Linear detection range (20 ?L sample): 0.5 to 65 U/L for 20 min reaction at 37C.
Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method: OD565nm
Samples: Biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media).
Species: All
Size: 100 tests
Detection Limit: 0.5 U/L
Shelf Life: 6 months
More Details: Malate dehydrogenase (MDH) (EC 1.1.1.37) is an enzyme which reversibly catalyzes the oxidation of L-malate to oxaloacetate in the presence of NAD. There are 2 isoforms in eukaryotic cells: MDH1 and MDH2. MDH1 found in the cytoplasm and plays a key part in the malate-aspartate shuttle for transporting malate into the mitochondria. MDH2 is a mitochondrial enzyme which participates in the TCA cycle that reversibly converts L-malate into oxaloacetate. Higher MDH activities are found in some neurodegenerative diseases such as Alzheimer?s disease. BioAssay Systems' non-radioactive, colorimetric MDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.