EnzyChrom D-Mannitol Assay Kit

I don't have the correct wavelength filter, will the assay still work?

Typically if you are within +/- 20 nanometers the assay will still work, but have slightly reduced sensitivity. Some assays have a narrower peak than others, call our tech support for a spectrum to verify.

I don't have a 96-well plate, will the assay kit work in a 384 well plate, cuvette, etc?

Yes, the assay can be used in any standard plate and cuvette. Simply adjust the total reaction volume to your container. For 384 well use 50 ?L, for a cuvette use 500 ?L or 1 mL.

Is the mannitol assay kit suitable for biological samples such as blood, urine, etc.?

The mannitol assay kit should be suitable for urine and blood samples as well as other biological samples. Some samples (e.g. cerebrospinal fluid) may contain mannitol under the detection limit (3 ?M); however, they should be detectable as long as the concentration is > 3 ?M.

How do I prepare the solid & liquid samples?

Solid Samples: chop or grind solid samples. Weigh 200 mg sample and homogenize (vortex or handheld homogenizer) in 800 ?L H₂O (Sample:H₂O = 1 mg: 4 ?L). Centrifuge homogenate at 14, 000g for 5-10 min. Transfer the clear supernatant into a clean tube. It is prudent to run a pilot test of the sample at different dilutions. Choose a dilution with the readings in the detection range of the standard curve for assays. Most samples can be stored at -80C if not assayed immediately.

Urine or Serum Samples: can be assayed direactly without dilution.

Can I store unused reagents for future use?

Yes, unused reagents can be stored according to the assay protocol. Repeated freeze/thaw cycles of reagents should be avoided. Since Assay Buffer is very light sensitive, always keeps buffer in the dark (amber bottle or tube) as much as possible for long term usage.

Can this manntiol assay kit detect L-Arabitol as well?

Unless the L-Arabitol is > 2M, this kit is unable to detect it.

Do I need to use a standard or standard curve with each assay run?

Yes, it is highly recommended.


For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.

ICE