EnzyChrom™ Ammonia Assay Kit

ICE

For quantitative determination of ammonia/ammonium and evaluation of drug effects on their metabolism. Key Features: High sensitivity and wide linear range. Use 20 µ L sample. Linear detection range 24 to 1000 µ M ammonia. Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of ammonia within 30 minutes. Method: OD340nm, or FL360/450nm. Samples: Serum, plasma, urine, saliva, cell culture etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 24 µ M.

Application: For quantitative determination of ammonia/ammonium concentration.
Key Features: High sensitivity and wide linear range. Use 20 ?L sample. Linear detection range 24 to 1000 ?M ammonia.
Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of ammonia within 30 minutes.
Method: OD340nm, or FL360/450nm
Samples: Serum, plasma, urine, saliva, cell culture etc
Species: All
Size: 100 tests
Detection Limit: 24 ?M
Shelf Life: 6 months
More Details: Ammonia (NH₃) or its ion form ammonium (NH₄⁺) is an important source of nitrogen for living systems. It is synthesized through amino acid metabolism and is toxic when present at high concentrations. In the liver, ammonia is converted to urea through the urea cycle. Elevated levels of ammonia in the blood (hyperammonemia) have been found in liver dysfunction (cirrhosis), while hypoammonemia has been associated with defects in the urea cycle enzymes (e.g. ornithine transcarbamylase). Simple, direct and automation-ready procedures for measuring NH₃ are popular in research and drug discovery. BioAssay Systems ammonia assay is designed to directly measure NH₃ and NH₄⁺. In this assay, NADH is converted to NAD⁺ in the presence of NH₃, ketoglutarate and glutamate dehydrogenase. The decrease in optical density at 340 nm or fluorescence intensity at ?ex/em = 360/450 nm is directly proportionate to the NH₃ concentration in the sample.