EnzyChrom™ Glutathione Reductase Kit

ICE

For quantitative determination of glutathione reductase activity. Key Features: Fast and sensitive. Linear detection range (20 µ L sample): 0.4 to 50 U/L for 20 min reaction. Detection Limit of 0.4 U/L. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD412nm. Samples: Plasma, serum, tissue and culture media etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.4 U/L.

Application: For quantitative determination of glutathione reductase activity.
Key Features: Fast and sensitive. Linear detection range (20 ?L sample): 0.4 to 50 U/L for 20 min reaction. Detection Limit of 0.4 U/L.
Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method: OD412nm
Samples: Plasma, serum, tissue and culture media etc
Species: All
Size: 100 tests
Detection Limit: 0.4 U/L
Shelf Life: 6 months
More Details: GLUTATHIONE REDUCTASE (GR) reduces oxidized glutathione (GSSG) to the reduced sulfhydryl form GSH which is an important cellular antioxidant. A high GSH/GSSG ratio is important for protection against oxidative stress. Thus, measurement of GR activity is used as indicator for oxidative stress. BioAssay Systems' non-radioactive, colorimetric GR assay is designed to accurately measure GR activity in biological samples with a method that utilizes Ellman?s method in which DTNB reacts with the GSH generated from the reduction of GSSG by the GR in a sample to form a yellow product (TNB^2-). The rate of change in the optical density, measured at 412 nm, is directly proportional to GR activity in the sample.