Human Glycated Hemoglobin A1c (HbA1c) ELISA Kit (CLIA)

Glycated Hemoglobin A1c

serum, plasma and erythrocyte lysates

2.44ug/mL

Chemiluminescent

Competitive Inhibition

Short term: 4CC; Long term: see manual.

8 months

This assay has high sensitivity and excellent specificity for detection of Glycated Hemoglobin A1c (HbA1c).

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Glycated Hemoglobin A1c (HbA1c). A competitive inhibition reaction is launched between biotin labeled Glycated Hemoglobin A1c (HbA1c) and unlabeled Glycated Hemoglobin A1c (HbA1c) (Standards or samples) with the pre-coated antibody specific to Glycated Hemoglobin A1c (HbA1c). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Glycated Hemoglobin A1c (HbA1c) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Glycated Hemoglobin A1c (HbA1c) level in the sample or standard.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.