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Human Glycated Hemoglobin A1c (HbA1c) ELISA Kit (CLIA)

Human Glycated Hemoglobin A1c (HbA1c) ELISA Kit (CLIA)

Manufacturer	Biomatik
Category	ELISA ELISA & Immunoassays ELISA Kits Immunoassay CLIA
Type	Elisa-Kit
Specific against	Human
Applications	CLIA
Amount	96T
Item no.	BM-EKU10875
eClass 6.1	32160605
eClass 9.0	32160605
Available
Format	96-Well Plate
Target Name	Glycated Hemoglobin A1c
Target Synonyms	Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc
Sample type	serum, plasma and erythrocyte lysates
Detection ra nge	5.86-1, 500ug/mL
Sensitivity	2.44ug/mL
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycated Hemoglobin A1c (HbA1c) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycated Hemoglobin A1c (HbA1c) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Intra-assay: CV<10%. Inter-Assay: CV<12%.
Detection Method	Chemiluminescent
Assay Time	2 hours
Assay Type	Competitive Inhibition
Shipping Condition	Ice packs
Storage	Short term: 4CC; Long term: see manual.
Precaution of Use	The Stop Solution is acidic. Do not allow to contact skin or eyes.
Expiry Date	8 months
UniProt ID	n/a
Specificity	This assay has high sensitivity and excellent specificity for detection of Glycated Hemoglobin A1c (HbA1c).
Cross Activity	This assay has high sensitivity and excellent specificity for detection of Glycated Hemoglobin A1c (HbA1c).
Test principle	The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Glycated Hemoglobin A1c (HbA1c). A competitive inhibition reaction is launched between biotin labeled Glycated Hemoglobin A1c (HbA1c) and unlabeled Glycated Hemoglobin A1c (HbA1c) (Standards or samples) with the pre-coated antibody specific to Glycated Hemoglobin A1c (HbA1c). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Glycated Hemoglobin A1c (HbA1c) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Glycated Hemoglobin A1c (HbA1c) level in the sample or standard.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 50 uL standard or sample to each well. And then add 50 uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37CC; 3. Aspirate and wash 3 times; 4. Add 100 uL prepared Detection Reagent B. Incubate 30 minutes at 37CC; 5. Aspirate and wash 5 times; 6. Add 100 uL Substrate Solution. Incubate 10 minutes at 37CC; 7. Read RLU value immediately.
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions	For Research Use Only. Not for use in diagnostic procedures, drug use, or for administration to humans or animals.
Quality Systems	The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Quality Assurance	Biomatik ELISA Kits are not typically pre-assembled as finished products due to limited shelf life. Before shipping, each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Detection Range, Sensitivity, and Precision. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Please always refer to the hard copy manual included in The kitbefore experiment.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96T

available

Delivery expected until 6/27/2024