Inositol Triphosphate (IP3) ELISA Kit (CLIA)

Ice packs

96-Well Plate

The Stop Solution is acidic. Do not allow to contact skin or eyes.

The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Inositol Triphosphate (IP3). A competitive inhibition reaction is launched between biotin labeled Inositol Triphosphate (IP3) and unlabeled Inositol Triphosphate (IP3) (Standards or samples) with the pre-coated antibody specific to Inositol Triphosphate (IP3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Inositol Triphosphate (IP3) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Inositol Triphosphate (IP3) level in the sample or standard.

2 hours

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inositol Triphosphate (IP3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inositol Triphosphate (IP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Intra-assay: CV<10%. Inter-Assay: CV<12%.

Chemiluminescent

This assay has high sensitivity and excellent specificity for detection of Inositol Triphosphate (IP3).