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Inositol Triphosphate (IP3) ELISA Kit (CLIA)

Inositol Triphosphate (IP3) ELISA Kit (CLIA)

Item no.	BM-EKU11562
Manufacturer	Biomatik
Amount	96 T
Category	ELISA ELISA & Immunoassays ELISA Kits Immunoassay CLIA
Type	Elisa-Kit
Applications	CLIA
Specific against	other
Sensitivity	46.4 pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	InsP3, Inositol 1,4,5-Trisphosphate, Triphosphoinositol
Available
Manufacturer - Targets	Inositol Triphosphate
Shipping Temperature	Ice packs
Storage Conditions	Short term: 4CC; Long term: see manual.
Manufacturer - Format	96-Well Plate
Restrictions	For Research Use Only. Not for use in diagnostic procedures, drug use, or for administration to humans or animals.
Precaution of use	The Stop Solution is acidic. Do not allow to contact skin or eyes.
Expiration	8 months
Quality Systems	The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Quality Assurance	Biomatik ELISA Kits are not typically pre-assembled as finished products due to limited shelf life. Before shipping, each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Detection Range, Sensitivity, and Precision. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Please always refer to the hard copy manual included in The kitbefore experiment.
Test Principles	The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Inositol Triphosphate (IP3). A competitive inhibition reaction is launched between biotin labeled Inositol Triphosphate (IP3) and unlabeled Inositol Triphosphate (IP3) (Standards or samples) with the pre-coated antibody specific to Inositol Triphosphate (IP3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Inositol Triphosphate (IP3) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Inositol Triphosphate (IP3) level in the sample or standard.
Sample Type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Time	2 hours
Assay Type	Competitive inhibition
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inositol Triphosphate (IP3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inositol Triphosphate (IP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Intra-assay: CV<10%. Inter-Assay: CV<12%.
Assay Procedure	1. Prepare all reagents, samples and standards; 2. Add 50 uL standard or sample to each well. And then add 50 uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37CC; 3. Aspirate and wash 3 times; 4. Add 100 uL prepared Detection Reagent B. Incubate 30 minutes at 37CC; 5. Aspirate and wash 5 times; 6. Add 100 uL Substrate Solution. Incubate 10 minutes at 37CC; 7. Read RLU value immediately.
Detection Method	Chemiluminescent
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Manufacturer - Specificity	This assay has high sensitivity and excellent specificity for detection of Inositol Triphosphate (IP3).
Manufacturer - Cross reactivity	This assay has high sensitivity and excellent specificity for detection of Inositol Triphosphate (IP3).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96 T

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Delivery expected until 7/31/2025

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