Hersteller |
Biomatik
|
Kategorie |
|
Typ |
Proteins Recombinant |
Specific against |
Human |
Menge |
50ug |
ArtNr |
BM-RPC20817-50ug |
Targets |
FBXW7 |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Lieferbar |
|
Gene Name |
FBXW7 |
Alternative Names |
Archipelago homolog1 Publication Short name: hAgo1 Publication F-box and WD-40 domain-containing protein 7Curated F-box protein FBX301 Publication SEL-101 Publication hCdc4 |
Uniprot |
Q969H0 |
Source |
in vitro E.coli expression system |
Expression Region |
1-707aa |
AA Sequence |
MNQELLSVGSKRRRTGGSLRGNPSSSQVDEEQMNR VVEEEQQQQLRQQEEEHTARNGEVVGVEPRPGGQN DSQQGQLEENNNRFISVDEDSSGNQEEQEEDEEHA GEQDEEDEEEEEMDQESDDFDQSDDSSREDEHTHT NSVTNSSSIVDLPVHQLSSPFYTKTTKMKRKLDHG SEVRSFSLGKKPCKVSEYTSTTGLVPCSATPTTFG DLRAANGQGQQRRRITSVQPPTGLQEWLKMFQSWS GPEKLLALDELIDSCEPTQVKHMMQVIEPQFQRDF ISLLPKELALYVLSFLEPKDLLQAAQTCRYWRILA EDNLLWREKCKEEGIDEPLHIKRRKVIKPGFIHSP WKSAYIRQHRIDTNWRRGELKSPKVLKGHDDHVIT CLQFCGNRIVSGSDDNTLKVWSAVTGKCLRTLVGH TGGVWSSQMRDNIIISGSTDRTLKVWNAETGECIH TLYGHTSTVRCMHLHEKRVVSGSRDATLRVWDIET GQCLHVLMGHVAAVRCVQYDGRRVVSGAYDFMVKV WDPETETCLHTLQGHTNRVYSLQFDGIHVVSGSLD TSIRVWDVETGNCIHTLTGHQSLTSGMELKDNILV SGNADSTVKIWDIKTGQCLQTLQGPNKHQSAVTCL QFNKNFVITSSDDGTVKLWDLKTGEFIRNLVTLES GGSGGVVWRIRASNTKLVCAVGSRNGTEETKLLVL DFDVDMK |
Sequence Info |
Full Length |
Tag Info |
N-terminal 6xHis-tagged |
Theoretical MW |
83.7 kDa |
Purity |
>90% as determined by SDS-PAGE. |
Storage Buffer |
Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Endotoxin Level |
Not tested |
Biological Activity |
Not tested |
Shipping Condition |
Ice packs |
Storage |
Short term: -20CC; Long term: -80CC. Minimize freeze and thaw cycles. |
Expiry Date |
1 year |
Restriction |
For Research Use Only. Not for use in diagnostic procedures, drug use, or for administration to humans or animals. |
Relevance |
Substrate recognition component of an SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination (PubMed:17434132). Identified substrates include cyclin-E (CCNE1 or CCNE2), JUN, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1 (PubMed:11565034, PubMed:12354302, PubMed:11585921, PubMed:15103331, PubMed:14739463, PubMed:17558397, PubMed:17873522, PubMed:22608923). Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation (PubMed:14739463). |
Function |
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination |
Subcellular location |
Isoform 1: Nucleus, nucleoplasm, SUBCELLULAR LOCATION: Isoform 2: Cytoplasm, SUBCELLULAR LOCATION: Isoform 3: Nucleus, nucleolus |
Tissue Specificity |
Isoform 1 is widely expressed. Isoform 3 is expressed in brain. |
Paythway |
Ubiquitinmediatedproteolysis |
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