Recombinant Human F-box/WD repeat-containing protein 7 (FBXW7)

Archipelago homolog1 Publication Short name: hAgo1 Publication F-box and WD-40 domain-containing protein 7Curated F-box protein FBX301 Publication SEL-101 Publication hCdc4

in vitro E.coli expression system

MNQELLSVGSKRRRTGGSLRGNPSSSQVDEEQMNR VVEEEQQQQLRQQEEEHTARNGEVVGVEPRPGGQN DSQQGQLEENNNRFISVDEDSSGNQEEQEEDEEHA GEQDEEDEEEEEMDQESDDFDQSDDSSREDEHTHT NSVTNSSSIVDLPVHQLSSPFYTKTTKMKRKLDHG SEVRSFSLGKKPCKVSEYTSTTGLVPCSATPTTFG DLRAANGQGQQRRRITSVQPPTGLQEWLKMFQSWS GPEKLLALDELIDSCEPTQVKHMMQVIEPQFQRDF ISLLPKELALYVLSFLEPKDLLQAAQTCRYWRILA EDNLLWREKCKEEGIDEPLHIKRRKVIKPGFIHSP WKSAYIRQHRIDTNWRRGELKSPKVLKGHDDHVIT CLQFCGNRIVSGSDDNTLKVWSAVTGKCLRTLVGH TGGVWSSQMRDNIIISGSTDRTLKVWNAETGECIH TLYGHTSTVRCMHLHEKRVVSGSRDATLRVWDIET GQCLHVLMGHVAAVRCVQYDGRRVVSGAYDFMVKV WDPETETCLHTLQGHTNRVYSLQFDGIHVVSGSLD TSIRVWDVETGNCIHTLTGHQSLTSGMELKDNILV SGNADSTVKIWDIKTGQCLQTLQGPNKHQSAVTCL QFNKNFVITSSDDGTVKLWDLKTGEFIRNLVTLES GGSGGVVWRIRASNTKLVCAVGSRNGTEETKLLVL DFDVDMK

N-terminal 6xHis-tagged

>90% as determined by SDS-PAGE.

Not tested

Ice packs

1 year

Substrate recognition component of an SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination (PubMed:17434132). Identified substrates include cyclin-E (CCNE1 or CCNE2), JUN, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1 (PubMed:11565034, PubMed:12354302, PubMed:11585921, PubMed:15103331, PubMed:14739463, PubMed:17558397, PubMed:17873522, PubMed:22608923). Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation (PubMed:14739463).

Isoform 1: Nucleus, nucleoplasm, SUBCELLULAR LOCATION: Isoform 2: Cytoplasm, SUBCELLULAR LOCATION: Isoform 3: Nucleus, nucleolus

Ubiquitinmediatedproteolysis