NucView® 488 Caspase-3 Substrate, 1mM in DMSO

Apoptosis, Viability Assays>Apoptosis Assays|Flow Cytometry|Enzyme Substrates>NucView® Caspase 3 Substrates|Cancer Research>NucView® Caspase-3 Substrates|Enzyme Substrates>Protease Substrates

NucView® 488 Caspase-3 Substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3/7 activity within live cells in real time. Unlike conventional caspase assays, NucView® 488 Caspase-3 Substrate detects caspase-3/7 activity within individual intact cells without inhibiting caspase activity. The substrate consists of a fluorogenic DNA dye and a DEVD substrate moiety specific for caspase-3/7. The substrate, which is both non-fluorescent and nonfunctional as a DNA dye, rapidly crosses cell membranes to enter the cytoplasm, where it is cleaved by caspase-3 to form a high-affinity DNA dye that stains the nucleus bright green. Thus, the NucView® 488 Caspase-3 Substrate is bi-functional, allowing detection of intracellular caspase-3/7 activity and visualization of changes in nuclear morphology during apoptosis. The fluorescent staining produced in response to caspase-3/7 activity is compatible with subsequent fixation and permeabilization for immunostaining.    NucView® 488 Caspase-3 Substrate, also is available as a 1 mM solution in PBS (phosphate-buffered saline) for use in cells that are sensitive to DMSO toxicity.     NucView® enzyme substrate technology is covered by U.S. patents.

Protect from light

Stable for at least 1 month

Dimethylsulfoxide >95%, CAS 67-68-5