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Fluo-4, AM ester, 50UG

Hersteller Biotium
Kategorie
Typ Reagents
Specific against other
Menge 10 ST
ArtNr B-50018
Targets ATF7IP
eClass 6.1 32160401
eClass 9.0 42051003
Lieferbar
Description
Membrane-permeant AM ester form of Fluo-4 that can enter into into cells via incubation. Fluo-4 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to Fluo-4 by endogenous esterases once the dye is inside the cells. Fluo-4 AM ester is often used to measure intracellular calcium in high throughput drug screening. Fluo-4 is an analog of Fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm that gives higher fluorescence signal. Fluo-4 has its absorption maximum at 494 nm, thus making it excitable by the argon-ion laser. Fluo-4 is essentially nonfluorescent without Ca2 present, but the fluorescence increases at least 100 times on Ca2 binding. Also, because Fluo-4 binds Ca2 more weakly (higher Kd) than do fura-2 and indo-1, it is more useful for measuring high transient Ca2 concentration during Ca2 spikes. Fluo-4 AM ester can be used to detect intracellular free calcium by fluorescence microscopy, flow cytometry, or fluorescence microplate reader. We also sell Fluo-4 as a membrane-impermeant pentapotassium salt version.
  • Orange red solid soluble in DMSO
  • Store -20C and protect from light, especially when in solution
  • Ex/Em of Ca2 €"bound form: 494/506 nm
  • Kd for Ca2 in buffer: ca.335 nM
  • C51H50F2N2O23
  • MW: 1096.95
  • [273221-67-3]
Shipping
blue ice
Storage Condition
F)-35C to -5C

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Menge: 10 ST
Lieferbar: In stock
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