Vergleich

Fluo-4, AM Ester, 10x50ug

ArtNr B-50018
Hersteller Biotium
CAS-Nr. 273221-67-3
Menge 10 x 50 UG
Kategorie
Typ Reagents
Specific against other
ECLASS 10.1 42051003
ECLASS 11.0 42051003
UNSPSC 12000000
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Category
Calcium Indicators &; Chelators
Shipping Temperature
Blue ice
Storage Conditions
-35°C to -5°C
Description
Membrane-permeant AM ester form of Fluo-4 that can enter into into cells via incubation. Fluo-4 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to Fluo-4 by endogenous esterases once the dye is inside the cells. Fluo-4 AM ester is often used to measure intracellular calcium in high throughput drug screening.    Fluo-4 is an analog of Fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm that gives higher fluorescence signal. Fluo-4 has its absorption maximum at 494 nm, thus making it excitable by the argon-ion laser. Fluo-4 is essentially nonfluorescent without Ca2 present, but the fluorescence increases at least 100 times on Ca2 binding. Also, because Fluo-4 binds Ca2 more weakly (higher Kd) than do fura-2 and indo-1, it is more useful for measuring high transient Ca2 concentration during Ca2 spikes.    Fluo-4 AM ester can be used to detect intracellular free calcium by fluorescence microscopy, flow cytometry, or fluorescence microplate reader.    We also sell Fluo-4 as a membrane-impermeant pentapotassium salt version. 
     
  • Orange red solid soluble in DMSO
  •  
  • Store -20°C and protect from light, especially when in solution
  •  
  • Ex/Em of Ca2 €“bound form: 494/506 nm
  •  
  • Kd for Ca2  in buffer: ~335 nM
  •  
  • C51H50F2N2O23
  •  
  • MW: 1096.95
  •  
  • [273221-67-3]
  •  
Product origin
Synthetic
Special handling for PI and label
Store with desiccant; Protect from light
Undated stability guarantee in PI
12 months
Stability at RT during shipping (protected from light)
Stable 4-5 days at RT as long as compound is dry
UNSPSC Commodity
41116113
UNSPSC Commodity Title
Cytology reagents or solutions or stains

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 10 x 50 UG
Lieferbar: In stock
lieferbar

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