Comparison

Fluo-4, AM Ester, 10x50ug

Item no. B-50018
Manufacturer Biotium
CASRN 273221-67-3
Amount 10 x 50 UG
Category
Type Reagents
Specific against other
ECLASS 10.1 42051003
ECLASS 11.0 42051003
UNSPSC 12000000
Shipping Condition Cool pack
Available
Manufacturer - Category
Calcium Indicators &; Chelators
Shipping Temperature
Blue ice
Storage Conditions
-35°C to -5°C
Description
Membrane-permeant AM ester form of Fluo-4 that can enter into into cells via incubation. Fluo-4 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to Fluo-4 by endogenous esterases once the dye is inside the cells. Fluo-4 AM ester is often used to measure intracellular calcium in high throughput drug screening.    Fluo-4 is an analog of Fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm that gives higher fluorescence signal. Fluo-4 has its absorption maximum at 494 nm, thus making it excitable by the argon-ion laser. Fluo-4 is essentially nonfluorescent without Ca2 present, but the fluorescence increases at least 100 times on Ca2 binding. Also, because Fluo-4 binds Ca2 more weakly (higher Kd) than do fura-2 and indo-1, it is more useful for measuring high transient Ca2 concentration during Ca2 spikes.    Fluo-4 AM ester can be used to detect intracellular free calcium by fluorescence microscopy, flow cytometry, or fluorescence microplate reader.    We also sell Fluo-4 as a membrane-impermeant pentapotassium salt version. 
     
  • Orange red solid soluble in DMSO
  •  
  • Store -20°C and protect from light, especially when in solution
  •  
  • Ex/Em of Ca2 €“bound form: 494/506 nm
  •  
  • Kd for Ca2  in buffer: ~335 nM
  •  
  • C51H50F2N2O23
  •  
  • MW: 1096.95
  •  
  • [273221-67-3]
  •  
Product origin
Synthetic
Special handling for PI and label
Store with desiccant; Protect from light
Undated stability guarantee in PI
12 months
Stability at RT during shipping (protected from light)
Stable 4-5 days at RT as long as compound is dry
UNSPSC Commodity
41116113
UNSPSC Commodity Title
Cytology reagents or solutions or stains

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 10 x 50 UG
Available: In stock
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