Anti-ARID1A Antibody

ArtNr	BOS-A00247
Hersteller	Boster
Menge	0.1 mg
Quantity options	0.1 mg 100 ul 100 ug/vial
Kategorie	Oncology Neuroscience Protein Aggregation, Clearance & Misfolding Antibodies By Cell Type Blastoma Carcinoma Hematology Lymphoma Melanoma Sarcoma Cell Biology Cell Movement Immunohistochemistry Western Blot ELISA Immunofluorescence Immunocytochemistry Primary Antibodies Cell Migration & Invasion By Organ Colorectal Cancer Immunostaining Immunochemistry
Typ	Antibody Polyclonal
Format	Liquid
Applikationen	WB, IF, ELISA, IHC-P
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	AT rich interactive domain 1A (SWI-like),RP1-50O24.1,B120,BAF250,BAF250a,BM029,C1orf4,ELD,hELD,MRD14,OSA1,hOSA1,P270,SMARCF1
Lieferbar
Manufacturer - Category	Primary Antibodies
Storage Conditions	ARID1A antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Molecular Weight	242045 MW
Clonality	Polyclonal
Application Details	ARID1A antibody can be used for detection of ARID1A by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Gene Name	ARID1A
Gene Full Name	AT rich interactive domain 1A (SWI-like)
Manufacturer - Specificity	ARID1A antibody is human, mouse and rat reactive. Multiple isoforms of ARID1A protein are known to exist.
Background	The ARID1A protein is a member of the SWI/SNF family, whose members are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. ARID1A is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin (1). It possesses a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. The C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that ARID1A confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions (2).
Immunogen	ARID1A antibody was raised against an 18 amino acid peptide near the amino terminus of human ARID1A. The immunogen is located within amino acids 580 - 630 of ARID1A.
Contents	ARID1A antibody is supplied in PBS containing 0.02% sodium azide.
Purification	ARID1A antibody is affinity chromatography purified via peptide column.
Concentration	1 mg/mL
Manufacturer - Research Category	Homeostasis, Signal Transduction
Protein Name	AT rich interactive domain 1A (SWI-like)
Protein Function	Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).
Subcellular Localization	Nucleus
Description	Boster Bio Anti-ARID1A Antibody (Catalog # A00247). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Tissue Specificity	Highly expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and PBL, and at a much lower level in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
Manufacturer - Cross Reactivity	ARID1A antibody is human, mouse and rat reactive. Multiple isoforms of ARID1A protein are known to exist.

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BOS-A00247-datasheet.pdf

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