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Anti-PUMA/BBC3 Antibody Picoband®

Anti-PUMA/BBC3 Antibody Picoband®

ArtNr	BOS-A04899-3-carrier-free
Hersteller	Boster
Menge	100 ug
Quantity options	100 ug/vial 10 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug
Kategorie	Oncology Antibodies By Cell Type Blastoma Carcinoma Hematology Leukemia Lymphoma Myeloma Cell Biology Cell Status Apoptosis Western Blot Autophagy Cardiovascular Research Ischemia & Reperfusion Injury Primary Antibodies By Organ Colorectal Cancer Testicular & Prostate Cancer
Typ	Antibody
Format	Lyophilized
Applikationen	WB
Specific against	Human (Homo sapiens)
Host	Rabbit
Isotype	IgG
Citations	1. Nakano K, Vousden KH (March 2001). PUMA, a novel proapoptotic gene, is induced by p53. Mol. Cell 7 (3): 683–94. 2. Han J, Flemington C, Houghton AB, Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T (September 2001). Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. Proc. Natl. Acad. Sci. U.S.A. 98 (20): 11318–23. 3. Yu, J.; Zhang, L.; Hwang, P. M.; Kinzler, K. W.; Vogelstein, B. : PUMA induces the rapid apoptosis of colorectal cancer cells. Molec. Cell 7: 673-682, 2001. 4. Nakano, K.; Vousden, K. H. : PUMA, a novel proapoptotic gene, is induced by p53. Molec. Cell 7: 683-694, 2001. 5. Chipuk, J. E.; Bouchier-Hayes, L.; Kuwana, T.; Newmeyer, D. D.; Green, D. R. : PUMA couples the nuclear and cytoplasmic proapoptotic function of p53. Science 309: 1732-1735, 2005.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Smoothelin,SMTN,SMSMO
Lieferbar
Manufacturer - Category	Primary Antibodies
Manufacturer - Conjugate / Tag	carrier-free
Storage Conditions	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Observed Molecular weight	18 kDa
Calculated Molecular weight	56903 MW
Clonality	Polyclonal
Application Details	Western blot, 0.25-0.5 μg/ml/ml, Human
Gene Name	BBC3
Gene Full Name	smoothelin
Background	The p53 upregulated modulator of apoptosis, or PUMA, is a pro-apoptotic member of the Bcl-2 protein family. The PUMA gene is located at 19q. PUMA transcript is contained within 4 exons, with the presumptive initiation codon in exon 2. The predicted 193-amino acid PUMA protein shares 91% amino acid identity with the murine sequence. Bcl-2 family members can form hetero- or homodimers, and they act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of PUMA is regulated by the tumor suppressor p53, and PUMA has been shown to be involved in p53-mediated apoptosis. Additionally, PUMA encodes 2 BH3 domain-containing proteins, PUMA-alpha and PUMA-beta, that are produced through the use of an alternative first exon and are induced in cells following p53 activation. Furthermore, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53.
Immunogen	A synthetic peptide corresponding to a sequence at the N-terminus of human PUMA/BBC3, which shares 95.7% amino acid (aa) sequence identity with mouse and rat BBC3.
Contents	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purification	Immunogen affinity purified.
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Manufacturer - Research Category	Vasculature
Protein Name	Vesicular acetylcholine transporter
Protein Function	Structural protein of the cytoskeleton.
Subcellular Localization	Cytoplasm, cytoskeleton. Exhibits a filamentous organization.
Description	Boster Bio Anti-PUMA/BBC3 Antibody Picoband® catalog # A04899-3. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity	Smooth muscle; contractile or vascular (for the long form).
Manufacturer - Cross Reactivity	No cross-reactivity with other proteins.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Arbeitsanleitung (Data Sheet) anfordern

BOS-A04899-3-carrier-free-datasheet.pdf

BOS-A04899-3-carrier-free-safety-datasheet.pdf