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Anti-Musashi 1/Msi1 Antibody Picoband®

Anti-Musashi 1/Msi1 Antibody Picoband®

ArtNr	BOS-A05052-2-Cy3
Hersteller	Boster
Menge	100 ug
Quantity options	100 ug/vial 10 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug
Kategorie	Oncology Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Antibodies By Cell Type Carcinoma Flow Cytometry Immunohistochemistry Western Blot ELISA Immunofluorescence Immunocytochemistry Primary Antibodies By Organ Lung Cancer Immunostaining Adhesion Molecule Markers Cytoskeleton Markers Immunochemistry
Typ	Primary Antibody
Format	Lyophilized
Applikationen	WB, FC, ELISA
Specific against	Human (Homo sapiens)
Host	Rabbit
Isotype	IgG
Konjugat/Tag	Cy3
Citations	1. Chavali, P. L., Stojic, L., Meredith, L. W., Joseph, N., Nahorski, M. S., Sanford, T. J., Sweeney, T. R., Krishna, B. A., Hosmillo, M., Firth, A. E., Bayliss, R., Marcelis, C. L., Lindsay, S., Goodfellow, I., Woods, C. G., Gergely, F. Neurodevelopmental protein Musashi-1 interacts with the Zika genome and promotes viral replication. Science 357: 83-88, 2017. 2. Good, P., Yoda, A., Sakakibara, S., Yamamoto, A., Imai, T., Sawa, H., Ikeuchi, T., Tsuji, S., Satoh, H., Okano, H. The human Musashi homolog 1 (MSI1) gene encoding the homologue of Musashi/Nrp-1, a neural RNA-binding protein putatively expressed in CNS stem cells and neural progenitor cells. Genomics 52: 382-384, 1998. 3. Siddall, N. A., McLaughlin, E. A., Marriner, N. L., Hime, G. R. The RNA-binding protein Musashi is required intrinsically to maintain stem cell identity. Proc. Nat. Acad. Sci. 103: 8402-8407, 2006.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	RNA-binding protein Musashi homolog 1,Musashi-1,MSI1
Lieferbar
Manufacturer - Category	Primary Antibodies
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular weight	39 kDa
Calculated Molecular weight	39125 MW
Clonality	Polyclonal
Application Details	Western blot, 0.25-0.5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human ELISA, 0.1-0.5μg/ml
Gene Name	MSI1
Gene Full Name	musashi RNA binding protein 1
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	RNA-binding protein Musashi homolog 1 is a protein that in humans is encoded by the MSI1 gene. This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13.
Immunogen	E.coli-derived human Musashi 1/Msi1 recombinant protein (Position: Q33-S347).
Contents	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Manufacturer - Research Category	Cell Type Marker, Cytoplasmic, Intracellular, Neural Stem Cells, Neurogenesis, Neurology Process, Neuroscience, Nuclear, Signaling Pathways, Stem Cells
Protein Name	RNA-binding protein Musashi homolog 1
Protein Function	RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U1-3AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
Subcellular Localization	Nucleus. Cytoplasm.
Description	Boster Bio Anti-Musashi 1/Msi1 Antibody Picoband® catalog # A05052-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity	Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.
Manufacturer - Cross Reactivity	No cross-reactivity with other proteins.

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Arbeitsanleitung (Data Sheet) anfordern

BOS-A05052-2-Cy3-datasheet.pdf

BOS-A05052-2-Cy3-safety-datasheet.pdf