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Anti-GFAP Antibody (Monoclonal, G-A-5)

Anti-GFAP Antibody (Monoclonal, G-A-5)

ArtNr	BOS-MA1045-10ug
Hersteller	Boster
Menge	10 ug
Quantity options	100 ug/vial 10 ug
Kategorie	Oncology Neuroscience Neural Lineage Markers Metabolism Diabetes Glucose Homeostasis Molecular Targets for Neuroscience Targets for Rodent Neurosciences Antibodies By Cell Type Blastoma Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies Neurodegeneration Markers Microglia Markers
Typ	Antibody Monoclonal
Format	Lyophilized
Applikationen	WB, IF, IHC, IHC-F
Clon	G-A-5
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus), Pig (Porcine, Sus scrofa domesticus)
Host	Mouse
Isotype	IgG1
Sensitivity	>5000 cells
Citations	1. Brenner, M.; Johnson, A. B.; Boespflug-Tanguy, O.; Rodriguez, D.; Goldman, J. E.; Messing, A. : Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nature Genet. 27: 117-120, 2001. 2. Rodriguez, D.; Gauthier, F.; Bertini, E.; Bugiani, M.; Brenner, M.; N'guyen, S.; Goizet, C.; Gelot, A.; Surtees, R.; Pedespan, J.-M.; Hernandorena, X.; Troncoso, M.; Uziel, G.; Messing, A.; Ponsot, G.; Pham-Dinh, D.; Dautigny, A.; Boespflug-Tanguy, O. : Infantile Alexander disease: spectrum of GFAP mutations and genotype-phenotype correlation. Am. J. Hum. Genet. 69: 1134-1140, 2001. Note: Erratum: Am. J. Hum. Genet. 69: 1413 only, 2001.
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Glial fibrillary acidic protein,GFAP,Gfap
Lieferbar
Manufacturer - Category	Primary Antibodies, Mouse Monoclonal Antibodies
Manufacturer - Isotype	Mouse IgG1
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Molecular Weight	50 kDa
Calculated Molecular weight	49957 MW
Clonality	Monoclonal
Application Details	Western blot, 0.5-1μg/ml, Human, mouse, pig, rat Immunohistochemistry (Paraffin-embedded Section), 0.4-1μg/ml, Human, pig, rat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, pig, rat, - Immunofluorescence, 2μg/ml, Rat
Gene Name	GFAP
Gene Full Name	Glial fibrillary acidic protein
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	Glial fibrillary acidic protein (GFAP) is an intermediate filament protein of 52Kda. GFAP gene is mapped to human 17q21. GFAP is a useful marker of astroglia in the brain. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease.
Immunogen	GFAP from pig spinal cord.
Contents	Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Purification	Ascites
Reconstitution	Add 1 ml of PBS buffer will yield a concentration of 100ug/ml.
Concentration	Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Manufacturer - Research Category	Cell Adhesion Proteins, Cell Type Marker, Cell Type Markers, Cytoskeleton, Cytoskeleton/ECM, Intermediate Filaments, Neural Stem Cells, Neuroscience, Signal Transduction, Stem Cells, Tags & Cell Markers
Protein Name	Glial fibrillary acidic protein
Protein Function	GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Subcellular Localization	Cytoplasm. Associated with intermediate filaments.
Description	Boster Bio Anti-GFAP Antibody (Monoclonal, G-A-5) catalog # MA1045. Tested in IF, IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Pig, Rat.
Tissue Specificity	Expressed in the cortex and hippocampus. Expression decreases following acute and chronic corticosterone treatment.
Sequence Similarities	Belongs to the intermediate filament family.

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BOS-MA1045-10ug-datasheet.pdf

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