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Anti-NF68 Nefl Antibody (Monoclonal, NR4) Europäischer Partner

ArtNr BOS-MA1070-10ug
Hersteller Boster
Menge 10 ug
Quantity options 100 ug/vial 10 ug
Kategorie
Typ Antibody Monoclonal
Format Lyophilized
Applikationen WB, IHC, IHC-F
Clon NR4
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus), Pig (Porcine, Sus scrofa domesticus)
Host Mouse
Isotype IgG1
Sensitivity <10 pg/ml
Citations 1. Zhu, Q.; Couillard-Despres, S.; Julien, J.-P. : Delayed maturation of regenerating myelinated axons in mice lacking neurofilaments. Exp. Neurol. 148: 299-316, 1997.
2. Jordanova, A.; De Jonghe, P.; Boerkoel, C. F.; Takashima, H.; De Vriendt, E.; Ceuterick, C.; Martin, J.-J.; Butler, I. J.; Mancias, P.; Papasozomenos, S. C.; Terespolsky, D.; Potocki, L.; Brown, C. W.; Shy, M.; Rita, D. A.; Tournev, I.; Kremensky, I.; Lupski, J. R.; Timmerman, V. : Mutations in the neurofilament light chain gene (NEFL) cause early onset severe Charcot-Marie-Tooth disease. Brain 126: 590-597, 2003.
3. Previtali, S. C.; Zerega, B.; Sherman, D. L.; Brophy, P. J.; Dina, G.; King, R. H. M.; Salih, M. M.; Feltri, L.; Quattrini, A.; Ravazzolo, R.; Wrabetz, L.; Monaco, A. P.; Bolino, A. : Myotubularin-related 2 protein phosphatase and neurofilament light chain protein, both mutated in CMT neuropathies, interact in peripheral nerve. Hum. Molec. Genet. 12: 1713-1723, 2003.
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias Neurofilament light polypeptide,NF-L,68 kDa neurofilament protein,Neurofilament triplet L protein,Nefl,Nf68,Nfl
Lieferbar
Manufacturer - Category
Primary Antibodies, Mouse Monoclonal Antibodies
Manufacturer - Isotype
Mouse IgG1
Storage Conditions
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Molecular Weight
72 kDa
Calculated Molecular weight
61335 MW
Clonality
Monoclonal
Application Details
Immunohistochemistry (Paraffin-embedded Section), 2-4μg/ml, Human, mouse, pig, rat
Immunohistochemistry (Frozen Section), 2-4μg/ml, Human, mouse, pig, rat, -
Western blot, 1-2μg/ml, Human, mouse, pig, rat
Gene Name
NEFL
Gene Full Name
Neurofilament light polypeptide
Manufacturer - Specificity
No cross reactivity with other proteins.
Background
Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD (NFL), 125 kD (NFM) and 200 kD (NFH) on SDS-gel electrophoresis. And they have a role in the maturation of regenerating myelinated axons. Neurofilament 68 (NF68), also called Neurofilament Protein, Light Chain (NFL). It is one of the most abundant cytoskeletal components of the neuron. Mutations in this gene were reported as a cause for autosomal dominant Charcot-Marie-Tooth type 2E (CMT2E) linked to chromosome 8p21. NFL was identified repeatedly in both screenings and found to interact with Myotubularin-related 2 gene, MTMR2 in both Schwann cells and neurons.
Immunogen
Pig spinal cord.
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Purification
Ascites
Reconstitution
Add 1 ml of PBS buffer will yield a concentration of 100ug/ml.
Concentration
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Manufacturer - Research Category
Cell Type Marker, Cytoskeleton, Cytoskeleton/ECM, Developmental Biology, Intermediate Filaments, Lineage Markers, Lineage Specification, Neurodegenerative Disease, Neurology Process, Neuron Marker, Neuroscience, Signal Transduction, Stem Cells
Protein Name
Neurofilament light polypeptide
Protein Function
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Subcellular Localization
Cytoplasm, myofibril, sarcomere. Cytoplasm, cytoskeleton.
Description
Boster Bio Anti-NF68 Nefl Antibody (Monoclonal, NR4) catalog # MA1070. Tested in IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Pig, Rat.
Tissue Specificity
Muscle specific. Located in the thin filament of striated muscle.
Sequence Similarities
Belongs to the intermediate filament family.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 10 ug
Lieferbar: In stock
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