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Anti-RIAM/APBB1IP Antibody Picoband® Europäischer Partner

ArtNr BOS-PA1960-iFluor647
Hersteller Boster
Menge 100 ug
Quantity options 100 ug/vial 10 ug 100 ug/vial 100 ug/vial 100 ug/vial 100 ug 100 ug 100 ug 100 ug/vial 100 ug/vial 100 ug/vial 100 ug/vial 100 ug
Kategorie
Typ Antibody Polyclonal
Format Lyophilized
Applikationen WB, FC, IF, IHC, ICC, IHC-F
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Rabbit
Isotype IgG
Sensitivity >5000 cells
Sequence Belongs to the MRL family.
Citations 1. Inagaki, T., Suzuki, S., Miyamoto, T., Takeda, T., Yamashita, K., Komatsu, A., Yamauchi, K., Hashizume, K. The retinoic acid-responsive proline-rich protein is identified in promyeloleukemic HL-60 cells. J. Biol. Chem. 278: 51685-51692, 2003.
2. Lafuente, E. M., van Puijenbroek, A. A. F. L., Krause, M., Carman, C. V., Freeman, G. J., Berezovskaya, A., Constantine, E., Springer, T. A., Gertler, F. B., Boussiotis, V. A. RIAM, an Ena/VASP and Profilin ligand, interacts with Rap1-GTP and mediates Rap1-induced adhesion. Dev. Cell 7: 585-595, 2004.
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Amyloid beta A4 precursor protein-binding family B member 1-interacting protein,APBB1-interacting protein 1,Proline-rich EVH1 ligand 1,PREL-1,Proline-rich protein 73,Rap1-GTP-interacting adapter molecule,RIAM,Retinoic acid-responsive proline-rich protein 1,RARP-1,APBB1IP,PREL1,RARP1,RIAM
Lieferbar
Manufacturer - Category
Primary Antibodies
Manufacturer - Conjugate / Tag
iFluor647
Storage Conditions
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular weight
110-120 kDa
Calculated Molecular weight
73183 MW
Clonality
Polyclonal
Application Details
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat
Immunocytochemistry , 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Gene Name
APBB1IP
Gene Full Name
Amyloid beta A4 precursor protein-binding family B member 1-interacting protein
Manufacturer - Specificity
No cross reactivity with other proteins.
Background
APBB1IP (APBB1-Interacting Protein), also called RIAM or RARP1, is a protein that in humans is encoded by the APBB1IP gene. By genomic sequence analysis, Lafuente et al. (2004) mapped the RIAM gene to chromosome 10p12.1. Using promoter-reporter gene assays, Inagaki et al. (2003) found that RARP1 suppressed transcription from AP1 and SRE sites, but not CRE sites, in all cell lines examined. The proline-rich regions of RARP1 suppressed AP1 transactivation. Lafuente et al. (2004) found that RIAM interacted with profilin and VASP, molecules that regulate actin dynamics, as well as with RAP1-GTP.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human APBB1IP, different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Purification
Immunogen affinity purified.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Manufacturer - Research Category
Developmental Families, Domain Families, Epigenetics and Nuclear Signaling, Lineage Markers, Lineage Specification, Neurogenesis, Neurology Process, Neuroscience, Oncoproteins, Oncoproteins/Suppressors, Stem Cells, Transcription, Transcription Factors
Protein Name
Amyloid beta A4 precursor protein-binding family B member 1-interacting protein
Protein Function
Appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling. Suppresses insulin- induced promoter activities through AP1 and SRE. Mediates Rap1- induced adhesion.
Subcellular Localization
Cell membrane; Peripheral membrane protein. Cell projection, lamellipodium. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Colocalizes with ENA/VASP proteins at lamellipodia tips and focal adhesions, and F- actin at the leading edge. At the membrane surface, associates, via the PH domain, preferentially with the inositol phosphates, PtdIns (5)P and PtdIns (3)P. This binding appears to be necessary for the efficient interaction of the RA domain to Ras-GTPases (By similarity).
Description
Boster Bio Anti-RIAM/APBB1IP Antibody catalog # PA1960. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity
Widely expressed with high expression in thymus, spleen, lymph node, bone marrow and peripheral leukocytes.
Sequence Similarities
Belongs to the MRL family.
Manufacturer - Alias
Amyloid beta A4 precursor protein-binding family B member 1-interacting protein, APBB1-interacting protein 1, Proline-rich EVH1 ligand 1, PREL-1, Proline-rich protein 73, Rap1-GTP-interacting adapter molecule, RIAM, Retinoic acid-responsive proline-rich protein 1, RARP-1, APBB1IP, PREL1, RARP1, RIAM
Manufacturer - Cross Reactivity
No cross-reactivity with other proteins

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
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