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Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA Antibody Picoband®

Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA Antibody Picoband®

ArtNr	BOS-PB9314-FITC
Hersteller	Boster
Menge	100 ug
Quantity options	100 ug/vial 10 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug
Kategorie	Oncology Neuroscience Neural Lineage Markers Metabolism Diabetes Cholesterol & Lipid Metabolism Regulation of Appetite Targets for Rodent Neurosciences Aging & Neurological Disorders Neural Development Antibodies By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Status Adenocarcinoma Mesothelioma Flow Cytometry Western Blot Autophagy Cardiovascular Research Proliferation Myocardial Infarction Primary Antibodies By Organ Breast Cancer Progenitor Markers
Typ	Antibody Polyclonal
Format	Lyophilized
Applikationen	WB, FC
Specific against	Human (Homo sapiens)
Host	Rabbit
Isotype	IgG
Konjugat/Tag	FITC
Sequence	Belongs to the PI3/PI4-kinase family.
Citations	1.Hiles I, Otsu M, Volinia S, Fry M, Gout I, Dhand R et al. (August 1992). Phosphatidylinositol 3-kinase: structure and expression of the 110 kd catalytic subunit. Cell 70 (3): 419–29. 2.Ma Y, Wei S, Lin Y, Lung J, Chang T, Whang-Peng J et al. (May 2000). PIK3CA as an oncogene in cervical cancer. Oncogene 19 (23): 2739–44. 3.Weigelt, B., Warne, P. H., Downward, J. PIK3CA mutation, but not PTEN loss of function, determines the sensitivity of breast cancer cells to mTOR inhibitory drugs. Oncogene 30: 3222-3233, 2011.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform,PI3-kinase subunit alpha,PI3K-alpha,PI3Kalpha,PtdIns-3-kinase subunit alpha,2.7.1.153,Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha,PtdIns-3-kinase subunit p110-alpha,p110alpha,Phosphoinositide-3-kinase catalytic alpha polypeptide,Serine/threonine protein kinase PIK3CA,2.7.11.1,PIK3CA
Lieferbar
Manufacturer - Category	Primary Antibodies
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular weight	124 kDa
Calculated Molecular weight	124284 MW
Clonality	Polyclonal
Application Details	Western blot, 0.1-0.5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
Gene Name	PIK3CA
Gene Full Name	Phosphatidylinositol 4, 5-bisphosphate 3-kinase catalytic subunit alpha isoform
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	Phosphatidylinositol-4, 5-bisphosphate 3-kinase, also called PIK3CA, is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. PIK3CA gene is mapped to 3q26.32. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate phosphatidylinositols (PtdIns), PtdIns4P andPtdIns(4, 5)P2. Recent evidence has shown that the PIK3CA gene is mutated in a range of human cancers. It has been found to be oncogenic and has been implicated in cervical cancers. PIK3CA mutations in breast cancer may be a predictive marker to guide the selection of patients who would benefit from mTOR inhibitor therapy. In addition to that, the presence of PIK3CA mutation may predict response to aspirin therapy for colorectal cancer, indicating power and promise of Molecular Pathological Epidemiology (MPE) approach as well as a complex interaction within the tumor microenvironment in this phenomenon.
Immunogen	E.coli-derived human PIK3CA recombinant protein (Position: H936-N1068). Human PIK3CA shares 98% amino acid (aa) sequence identity with mouse PIK3CA.
Contents	Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Protein Name	Phosphatidylinositol 4, 5-bisphosphate 3-kinase catalytic subunit alpha isoform
Protein Function	Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4- phosphate) and PtdIns (4, 5)P2 (Phosphatidylinositol 4, 5- bisphosphate) to generate phosphatidylinositol 3, 4, 5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation in breast cancer cells through the PDPK1- AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Has also serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS.
Subcellular Localization	Cytoplasm. Nucleus. Mostly nuclear; oxidative stress promotes cytoplasmic localization.
Description	Boster Bio Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA Antibody Picoband® catalog # PB9314. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity	Expressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in brain tissue.
Sequence Similarities	Belongs to the PI3/PI4-kinase family.
Manufacturer - Alias	Phosphatidylinositol 4, 5-bisphosphate 3-kinase catalytic subunit alpha isoform, PI3-kinase subunit alpha, PI3K-alpha, PI3Kalpha, PtdIns-3-kinase subunit alpha, 2.7.1.153, Phosphatidylinositol 4, 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, PtdIns-3-kinase subunit p110-alpha, p110alpha, Phosphoinositide-3-kinase catalytic alpha polypeptide, Serine/threonine protein kinase PIK3CA, 2.7.11.1, PIK3CA
Manufacturer - Cross Reactivity	No cross-reactivity with other proteins

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BOS-PB9314-FITC-datasheet.pdf

BOS-PB9314-FITC-safety-datasheet.pdf