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Human recombinant TNF alpha protein, GMP

Human recombinant TNF alpha protein, GMP

ArtNr	BOS-PROTP01375-9-100ug
Hersteller	Boster
Menge	100 ug
Quantity options	100 ug 1 mg
Kategorie	Oncology Neuroscience Neuroinflammation Metabolism Diabetes By Cell Type Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis ELISA Immunostaining Cell Culture
Format	Lyophilized
Applikationen	ELISA, Cell Culture
Specific against	Human (Homo sapiens)
Konjugat/Tag	HIS
Sequence	MVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL with polyhistidine tag at the C-terminus.
Alias	TNFSF2,Cachectin,Differentiation-inducing factor (DIF),Necrosin,Cytotoxin,TNS_x0002_F1A
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Manufacturer - Category	Recombinant Proteins
Manufacturer - Conjugate / Tag	His Tag (C-term)
Storage Conditions	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Molecular Weight	The protein has a calculated MW of 18.3 kDa. The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).
Manufacturer - Format	Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.
Calculated Molecular weight	The protein has a calculated MW of 18.3 kDa.The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).
Gene Name	TNF
Purification	>97% as determined by SDS-PAGE analysis.
Reconstitution	Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution at RT for at least 20 min to ensure sufficient re-dissolved.
Description	Tumor necrosis factor alpha (TNF alpha) stimulates the acute phase of the immune response. In response to a pathogen, TNF alpha is one of the first to be released and can apply its effects in many organs. TNF alpha stimulates the release of corticotropic releasing hormone, suppresses appetite, and induces fever, in the hypothalamus. TNF increase vasodilation and loss of vascular permeability, it helps recruit lymphocyte, neutrophil, and monocyte to the inflammation site by regulating chemokine release.
Bioactivity/Biological Activity	Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED₅₀ for this effect is < 0.1 ng/mL. The specific activity of recombinant human TNF alpha is approximately ≧ 1 x 10⁷ IU/mg, which is calibrated against the human TNF Alpha WHO International Standard (NIBSC code: 12/154).
Endotoxin level	<0.05 EU per 1 μg of the protein by the LAL method.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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BOS-PROTP01375-9-100ug-datasheet.pdf

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Einfache Bestellung Periodische Bestellung

Menge: 100 ug

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Lieferung vsl. bis 04.12.2025