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Human recombinant ENO1 protein, AF

Human recombinant ENO1 protein, AF

ArtNr	BOS-PROTP06733-2-20ug
Hersteller	Boster
Menge	20 ug
Quantity options	100 ug 20 ug 5 ug
Kategorie	Cell Culture
Format	Lyophilized
Applikationen	Cell Culture
Specific against	Human (Homo sapiens)
Konjugat/Tag	HIS
Sequence	SILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGK
Alias	enolase 1,NNE,PPH,MPB1,ENO1L1,HEL-S-17
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Manufacturer - Category	Recombinant Proteins
Manufacturer - Conjugate / Tag	His Tag (N-term)
Storage Conditions	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Molecular Weight	The protein has a calculated MW of 47.85 kDa. The protein migrates as 55 kDa under reducing condition (SDS-PAGE analysis).
Manufacturer - Format	The protein was lyophilized from a 0.2 µm filtered solution containing 7 mM MgSO4 in 1X PBS, pH 7.2 If you have any concerns or special requirements, please confirm with us.
Calculated Molecular weight	The protein has a calculated MW of 47.85 kDa.The protein migrates as 55 kDa under reducing condition (SDS-PAGE analysis).
Gene Name	ENO1
Purification	>98% as determined by SDS-PAGE.
Reconstitution	Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Description	Enolase 1 (ENO1) also named alpha-enolase which is a glycolytic enzyme. It could convert the NAD+ to NADH by producing two ATP. ENO1 is a homodimer which is composed by two isozymes of enolase (2 α, 2 ν, or 2 β). It also plays an important role in cancer model which can promote tumor cell proliferation and migration by the PI3K signaling pathway. ENO1 is a biomarker of prognostic and diagnostic cancer.
Bioactivity/Biological Activity	Testing in process
Endotoxin level	<0.1 EU per 1 μg of the protein by the LAL method.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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BOS-PROTP06733-2-20ug-datasheet.pdf

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Menge: 20 ug

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Lieferung vsl. bis 04.12.2025