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Human recombinant VEGF121 (Vascular endothelial growth factor 121) protein, AF

Human recombinant VEGF121 (Vascular endothelial growth factor 121) protein, AF

ArtNr	BOS-PROTP15692-17-20ug
Hersteller	Boster
Menge	20 ug
Quantity options	100 ug 20 ug 5 ug
Kategorie	Oncology By Cell Type Carcinoma Cell Biology Cell Culture Cytokines Adenocarcinoma By Organ Bladder Cancer Cell Culture
Format	Lyophilized
Applikationen	Cell Culture
Specific against	Human (Homo sapiens)
Konjugat/Tag	HIS
Sequence	MAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENCDKPRR with polyhistidine tag at the C-terminus.
Alias	VPF
Lieferbar
Manufacturer - Category	Recombinant Proteins
Manufacturer - Conjugate / Tag	His Tag (C-term)
Storage Conditions	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Molecular Weight	The protein has a calculated MW of 15.00 kDa. The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).
Manufacturer - Format	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
Calculated Molecular weight	The protein has a calculated MW of 15.00 kDa.The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).
Gene Name	VEGFA
Purification	>95% as determined by SDS-PAGE.
Reconstitution	Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Description	Vascular Endothelial Growth Factors 121(VEGF121) is one of five VEGF splice variants of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length. VEGF121 is the only VEGF which lacks heparin-binding activity and freely diffusible. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1 /KDR) on endothelial cells to activate signal transduction and regulate both physiological and pathological angiogenesis.
Bioactivity/Biological Activity	Measure by its ability to induce proliferation in HUVEC cells. The ED₅₀ for this effect is <2.5 ng/mL.
Endotoxin level	<0.1 EU per 1 μg of the protein by the LAL method.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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BOS-PROTP15692-17-20ug-datasheet.pdf

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Menge: 20 ug

lieferbar

Lieferung vsl. bis 04.12.2025