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Human recombinant TRAIL (TNF-related apoptosis-inducing ligand) protein, AF Europäischer Partner

ArtNr BOS-PROTP50591-6-5ug
Hersteller Boster
Menge 5 ug
Quantity options 100 ug 20 ug 5 ug
Kategorie
Format Lyophilized
Applikationen Cell Culture
Specific against Human (Homo sapiens)
Konjugat/Tag HIS
Sequence MRERGPQRVAAHITGTRGRSNTLSSPNSKNEKALGRKINSWESSRSGHSFLSNLHLRNGELVIHEKGFYYIYSQTYFRFQEEIKENTKNDKQMVQYIYKYTSYPDPILLMKSARNSCWSKDAEYGLYSIYQGGIFELKENDRIFVSVTNEHLIDMDHEASFFGAFLVG with polyhistidine tag at the C-terminus.
Alias TNFSF10,Apo2 Ligand,TL2,Apo2L,CD253
Lieferbar
Manufacturer - Category
Recombinant Proteins
Manufacturer - Conjugate / Tag
His Tag (C-term)
Storage Conditions
Lyophilized protein should be stored at -20°C for 1 year.
Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1%
BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Molecular Weight
The protein has a calculated MW of 20.33 kDa.
The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).
Manufacturer - Format
The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
Calculated Molecular weight
The protein has a calculated MW of 20.33 kDa.The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).
Gene Name
TNFSF10
Purification
>98% as determined by SDS-PAGE.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Description
Tumor necrosis factor?associated apoptosis?inducing ligand (TRAIL) is a type II membrane protein of the tumor necrosis factor (TNF) family members, moreover, expressed in many adult tissues including the thymus, prostate, colon, ovary and lung. TRAIL is a 19kDa protein containing 281 residues. TRAIL to induce apoptosis in human breast carcinoma cells (MCF7) and human epitheloid carcinoma (HeLa) cell lines, by activate two death receptors of DR4 and DR5 or two decoy receptors DcR1 and DcR2.
Bioactivity/Biological Activity
Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D.
The ED₅₀ for this effect is 10.4-15.4 ng/mL.
Endotoxin level
<0.1 EU per 1 μg of the protein by the LAL method.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 5 ug
Lieferbar: In stock
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Lieferung vsl. bis 04.12.2025 

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