Hersteller |
Boster
|
Kategorie |
|
Typ |
Antibody Polyclonal |
Specific against |
Human |
Isotype |
IgG |
Format |
Lyophilized |
Applikationen |
WB, FC, IF, IHC, ICC, IHC-F |
Menge |
100 ug/vial |
Host |
Rabbit |
ArtNr |
BOS-A01267 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Manufacturers Product Category |
Primary Antibodies, Rabbit Polyclonal Antibodies |
Manufacturers Research Category |
Apoptosis, Calcium Channels, Calcium Signaling, Cancer, Cardiovascular, Cell Biology, Cell Death, Cholesterol Metabolism, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Metabolism Processes, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Pathways and Processes, Signal Transduction, Signaling Pathway |
Short Description |
Boster Bio Anti-Diazepam Binding Inhibitor/DBI Antibody Picoband™ catalog # A01267. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. |
Description |
Boster Bio Anti-Diazepam Binding Inhibitor/DBI Antibody Picoband™ catalog # A01267. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. |
Background |
Acyl-CoA-binding protein is a protein that in humans is encoded by the DBI gene. This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene. |
Gene Name |
DBI |
Application Details |
Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human |
Application Notes |
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. |
Clonality |
Polyclonal |
Concentration |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Contents |
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Immunogen |
E. coli-derived human DBI recombinant protein (Position: S2-I87). Human DBI shares 77.9% amino acid (aa) sequence identity with both mouse and rat DBI. |
Purification |
Immunogen affinity purified. |
Cross-reactivity |
No cross-reactivity with other proteins |
Storage |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Reconstitution |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene Full Name |
Acyl-CoA-binding protein |
Molecular Weight |
10044 MW |
Protein Function |
Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor. |
Subcellular Localization |
Endoplasmic reticulum. Golgi apparatus. Golgi localization is dependent on ligand binding (PubMed:17953517).. |
Tissue Specificity |
Isoform 1 is ubiquitous, with a moderate expression level. Isoform 2 is ubiquitous with high level in liver and adipose tissue. Isoform 3 is ubiquitous with strong expression in adipose tissue and heart.. |
Protein Name |
Acyl-CoA-binding protein |
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