Hersteller |
Boster
|
Kategorie |
|
Typ |
Antibody Polyclonal |
Specific against |
Human |
Isotype |
IgG |
Format |
Lyophilized |
Applikationen |
WB, FC |
Menge |
100 ug/vial |
Host |
Rabbit |
ArtNr |
BOS-PB9154 |
Targets |
ICOSLG , L1CAM, CD279, CD274, CD273 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Manufacturers Product Category |
Primary Antibodies, Rabbit Polyclonal Antibodies |
Manufacturers Research Category |
Autoimmune, Cancer, Cell Type Markers, Immune System Diseases, Immunology, Tumor Antigens, Tumor Biomarkers, Tumor Immunology, Tumor-Associated Antigens |
Short Description |
Boster Bio Anti-PD-L1/CD274 Antibody Picoband™ catalog # PB9154. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. |
Description |
Boster Bio Anti-PD-L1/CD274 Antibody Picoband™ catalog # PB9154. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. |
Background |
Programmed death-ligand 1 (PD-L1) also known as CD274 or B7-H1 is a protein that in humans is encoded by the CD274 gene. It is mapped to 9p24.1. PD-L1 is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. It has been concluded that upregulation of PD-L1 on tumor MDCs downregulates T-cell immunity and that PD-L1 blockade may represent an approach for cancer immunotherapy. PD-L1 can provide positive costimulatory signals for innate and adaptive immunity and for protection against intracellular bacterial infection. What’s more, It has been found that PD1/PDL1 pathway may be a good target for restoring antitumor immunity in ovarian cancer. |
Gene Name |
CD274 |
Application Details |
Western blot, 0.25-0.5μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human |
Application Notes |
WB: The detection limit for PD-L1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. |
Clonality |
Polyclonal |
Concentration |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Contents |
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4. |
Immunogen |
E.coli-derived human PD-L1 recombinant protein (Position: E45-T290). Human PD-L1 shares 69% amino acid (aa) sequence identity with mouse PD-L1. |
Purification |
Immunogen affinity purified. |
Cross-reactivity |
No cross-reactivity with other proteins |
Storage |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Reconstitution |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene Full Name |
Programmed cell death 1 ligand 1 |
Molecular Weight |
33275 MW |
Protein Function |
Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.. |
Subcellular Localization |
Isoform 1: Cell membrane; Single-pass type I membrane protein. |
Tissue Specificity |
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.. |
Protein Name |
Programmed cell death 1 ligand 1 |
Sequence Similarities |
Belongs to the immunoglobulin superfamily. BTN/MOG family. |
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