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Anti-Muscarinic Acetylcholine Receptor 2/CHRM2 Antibody

Anti-Muscarinic Acetylcholine Receptor 2/CHRM2 Antibody

Hersteller	Boster
Kategorie	Neuroscience Neural Signaling Assisted reproductive technologies Embryo manipulation Antibodies Immunohistochemistry Western Blot Primary Antibodies
Typ	Antibody Polyclonal
Specific against	Human, Mouse, Rat
Isotype	IgG
Format	Lyophilized
Applikationen	WB, IHC
Menge	100 ug/vial
Host	Rabbit
ArtNr	BOS-PA1325-1
Targets	CHRM2
eClass 6.1	32160702
eClass 9.0	32160702
Lieferbar
Manufacturers Product Category	Primary Antibodies, Rabbit Polyclonal Antibodies
Short Description	Boster Bio Anti-Muscarinic Acetylcholine Receptor 2/CHRM2 Antibody catalog # PA1325-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Description	Boster Bio Anti-Muscarinic Acetylcholine Receptor 2/CHRM2 Antibody catalog # PA1325-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Background	The muscarinic acetylcholine receptor M2, also known as the cholinergic receptor, muscarinic 2, is a muscarinic acetylcholine receptor that in humans is encoded by the CHRM2 gene. The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene.
Gene Name	CHRM2
Application Details	Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Application Notes	WB: The detection limit for CHRM2 is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
Predicted Reactivity	Hamster
Clonality	Polyclonal
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Contents	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen	A synthetic peptide corresponding to a sequence at the C-terminus of human Muscarinic Acetylcholine Receptor 2, identical to the related rat and mouse sequences.
Purification	Immunogen affinity purified.
Cross-reactivity	No cross-reactivity with other proteins
Storage	Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Gene Full Name	Muscarinic acetylcholine receptor M2
Molecular Weight	51715 MW
Protein Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol..
Subcellular Localization	Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Phosphorylation in response to agonist binding promotes receptor internalization..
Protein Name	Muscarinic acetylcholine receptor M2

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