Anti-Orai1 Antibody Picoband®

Primary Antibodies

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

32668 MW

Western blot, 0.1-0.5?g/ml, Human, Mouse, Rat

Calcium release-activated calcium channel protein 1

ORAI1 (ORAI calcium release-activated calcium modulator 1), also known as CRACM1, TMEM142A, Calcium release-activated calcium channel protein 1, Protein orai-1, Transmembrane protein 142A, FLJ14466, is a calcium selective  ion channel  that in humans is encoded by the  ORAI1  gene. Orai1 channels play an important role in the activation of T-lymphocytes. The loss of function mutation of Orai1 causes  severe combined immunodeficiency (SCID) in humans. The mammalian orai family has two additional homologs, orai2 and orai3. Orai proteins share no homology with any other ion channel family of any other known proteins. They have 4  transmembrane  domains and form tetramers.  Prakriya et al. (2006) showed that ORAI1 is a PM protein, and that CRAC channel function is sensitive to mutation of 2 conserved acidic residues in the transmembrane segments. Glu106-to-asp (E106D) and glu190-to-gln (E190Q) substitutions in transmembrane helices 1 and 3, respectively, diminished calcium ion influx, increased current carried by monovalent cations, and rendered the channel permeable to cesium ion.  Prakriya et al. (2006)  showed that ORAI1 is a PM protein, and that CRAC channel function is sensitive to mutation of 2 conserved acidic residues in the transmembrane segments.

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2mg Na2HPO4.

Add 0.2 ml of distilled water will yield a concentration of 500 ug/ml.

Adaptive Immunity, Calcium Channels, Calcium Signaling, Immunology, Non-CD, Signal Transduction, Signaling Pathway, T Cells

Ca (2+) release-activated Ca (2+) (CRAC) channel subunit which mediates Ca (2+) influx following depletion of intracellular Ca (2+) stores and channel activation by the Ca (2+) sensor, STIM1. CRAC channels are the main pathway for Ca (2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.

Boster Bio Anti-Orai1 Antibody catalog # PA1857. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Belongs to the Orai family.