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Anti-RGS14 Antibody Picoband®

Anti-RGS14 Antibody Picoband®

ArtNr	BOS-PA2163
Hersteller	Boster
Menge	100 ug/vial
Kategorie	Neuroscience Molecular Targets for Neuroscience Targets for Rodent Neurosciences Neural Signaling Antibodies Cell Biology Cytoskeleton Western Blot Primary Antibodies small GTPases Ras Subfamily Rap Subfamily
Typ	Antibody Polyclonal
Format	Lyophilized
Applikationen	WB
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Sensitivity	<10pg/ml
Citations	1. Kimple, R. J., Kimple, M. E., Betts, L., Sondek, J., Siderovski, D. P. Structural determinants for GoLoco-induced inhibition of nucleotide release by G-alpha subunits. Nature 416: 878-881, 2002. 2. Traver, S., Bidot, C., Spassky, N., Baltauss, T., de Tand, M.-F., Thomas, J.-L., Zalc, B., Janoueix-Lerosey, I., de Gunzburg, J. RGS14 is a novel Rap effector that preferentially regulates the GTPase activity of G-alpha-0. Biochem. J. 350: 19-29, 2000.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Regulator of G-protein signaling 14;RGS14;RGS14;
Similar products	Highly similar to rap1/rap2 interacting protein antibody, OTTHUMP00000223586 antibody, OTTHUMP00000223587 antibody, Regulation of G protein signaling 14 antibody, Regulator of G protein signaling 14 antibody, Regulator of G protein signalling 14 antibody, Regulator of G-protein signaling 14 antibody, RGS 14 antibody, RGS14 antibody, RGS14_HUMAN antibody
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Manufacturer - Category	Primary Antibodies
Manufacturer - Isotype	Rabbit IgG
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight	15 kDa
Calculated Molecular weight	61447 MW
Clonality	Polyclonal
Application Details	Western blot, 0.1-0.5?g/ml, Human, Rat, Mouse
Manufacturer - Gene Name	RGS14
Gene Full Name	Regulator of G-protein signaling 14
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	Regulator of G-protein signaling 14, also called RGS14, is a protein that in humans is encoded by the RGS14 gene. This gene encodes a member of the regulator of G-protein signaling family. By genomic sequence analysis, this gene is mapped to chromosome 5q35.3. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal.
Immunogen	A synthetic peptide corresponding to a sequence at the C-terminus of human RGS14, identical to the related mouse and rat sequences.
Contents	Each vial contains 5 mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Manufacturer - Research Category	G Protein Signaling, Nucleotide Messenger, Second Messenger, Signal Transduction, Signaling Pathway
Protein Name	Regulator of G-protein signaling 14
Protein Function	Acts as a regulator of G protein signaling (RGS). Modulates G protein alpha subunits nucleotide exchange and hydrolysis activities by functioning either as a GTPase-activating protein (GAP), thereby driving G protein alpha subunits into their inactive GDP-bound form, or as a GDP-dissociation inhibitor (GDI). Confers GDI activity on G (i) alpha subunits GNAI1 and GNAI3, but not G (o) alpha subunit GNAO1 and G (i) alpha subunit GNAI2. Confers GAP activity on G (o) alpha subunit GNAI0 and G (i) alpha subunits GNAI2 and GNAI3. May act as a scaffold integrating G protein and Ras/Raf MAPkinase signaling pathways. Inhibits platelet-derived growth factor (PDGF)-stimulated ERK1/ERK2 phosphorylation; a process depending on its interaction with HRAS and that is reversed by G (i) alpha subunit GNAI1. Acts as a positive modulator of microtubule polymerisation and spindle organization through a G (i)-alpha-dependent mechanism. Plays a role in cell division. Probably required for the nerve growth factor (NGF)-mediated neurite outgrowth. May be involved in visual memory processing capacity and hippocampal-based learning and memory.
Subcellular Localization	Nucleus. Nucleus, PML body. Cytoplasm. Membrane. Cell membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Cell projection, dendrite. Cell projection, dendritic spine. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Associates with the perinuclear sheaths of microtubules (MTs) surrounding the pronuclei, prior to segregating to the anastral mitotic apparatus and subsequently the barrel-shaped cytoplasmic bridge between the nascent nuclei of the emerging 2-cell embryo. Localizes to a perinuclear compartment near the microtubule- organizing center (MTOC). Expressed in the nucleus during interphase and segregates to the centrosomes and astral MTs during mitosis. Relocalizes to the nucleus in PML nuclear bodies in response to heat stress. Colocalizes with RIC8A in CA2 hippocampal neurons. Localizes to spindle poles during metaphase. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner. Recruited from the cytosol to the plasma membrane by the inactive GDP-bound forms of G (i) alpha subunits GNAI1 and GNAI3. Recruited from the cytosol to membranes by the active GTP-bound form of HRAS. Colocalizes with G (i) alpha subunit GNAI1 and RIC8A at the plasma membrane. Colocalizes with BRAF and RAF1 in both the cytoplasm and membranes (By similarity).
Description	Boster Bio Anti-RGS14 Antibody catalog # PA2163. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Tissue Specificity	Highly expressed in brain, skeletal muscle and kidney and less strongly in heart, placenta, lung and liver.
Sequence Similarities	Contains 1 GoLoco domain.

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